Identification of region-specific transcription factor genes in the adult mouse brain by medium-scale real-time RT-PCR

Harukazu Suzuki; Rie Okunishi; Wataru Hashizume; Shintaro Katayama; Noriko Ninomiya; Naoki Osato; Kenjiro Sato; Mari Nakamura; Juri Iida; Mutsumi Kanamori; Yoshihide Hayashizaki

doi:10.1016/j.febslet.2004.07.068

Identification of region-specific transcription factor genes in the adult mouse brain by medium-scale real-time RT-PCR

Harukazu Suzuki^*, Rie Okunishi, Wataru Hashizume, Shintaro Katayama, Noriko Ninomiya, Naoki Osato, Kenjiro Sato, Mari Nakamura, Juri Iida, Mutsumi Kanamori, Yoshihide Hayashizaki

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

12 Citations (Scopus)

Abstract

We established a medium-scale real-time RT-PCR system focusing on transcription factors and applied it to their expression profiles in the adult mouse 11 brain regions (http://genome.gsc.riken.jp/qRT-PCR/). Almost 90% of the examined genes showed significant expression in at least one region. We successfully extracted 179 region-specific genes by clustering analysis. Interestingly, the transcription factors involved in the development of the pituitary were still expressed in the adult pituitary, suggesting that they also play important roles in maintenance of the pituitary. These results provide unique molecular markers that may account for the molecular basis of the unique functions of specific brain regions.

Original language	English
Pages (from-to)	214-218
Number of pages	5
Journal	FEBS Letters
Volume	573
Issue number	1-3
DOIs	https://doi.org/10.1016/j.febslet.2004.07.068
Publication status	Published - 2004 Aug 27
Externally published	Yes

Keywords

Real-time RT-PCR
Region-specific
Transcription factor

ASJC Scopus subject areas

Biophysics
Structural Biology
Biochemistry
Molecular Biology
Genetics
Cell Biology

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10.1016/j.febslet.2004.07.068

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title = "Identification of region-specific transcription factor genes in the adult mouse brain by medium-scale real-time RT-PCR",

abstract = "We established a medium-scale real-time RT-PCR system focusing on transcription factors and applied it to their expression profiles in the adult mouse 11 brain regions (http://genome.gsc.riken.jp/qRT-PCR/). Almost 90% of the examined genes showed significant expression in at least one region. We successfully extracted 179 region-specific genes by clustering analysis. Interestingly, the transcription factors involved in the development of the pituitary were still expressed in the adult pituitary, suggesting that they also play important roles in maintenance of the pituitary. These results provide unique molecular markers that may account for the molecular basis of the unique functions of specific brain regions.",

keywords = "Real-time RT-PCR, Region-specific, Transcription factor",

author = "Harukazu Suzuki and Rie Okunishi and Wataru Hashizume and Shintaro Katayama and Noriko Ninomiya and Naoki Osato and Kenjiro Sato and Mari Nakamura and Juri Iida and Mutsumi Kanamori and Yoshihide Hayashizaki",

note = "Funding Information: We thank Dr. K. Polland (UCSC) for providing the HOPACH program and A. Hasegawa for technical assistance. We also thank the members of the Laboratory for Genome Exploration Research Group. This study was supported by MEXT Research Grants for the RIKEN Genome Exploration Research Project, for Advanced and Innovational Research Program in Life Science and for National Project on Genome Network Analysis to Y.H.",

year = "2004",

month = aug,

day = "27",

doi = "10.1016/j.febslet.2004.07.068",

language = "English",

volume = "573",

pages = "214--218",

journal = "FEBS Letters",

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T1 - Identification of region-specific transcription factor genes in the adult mouse brain by medium-scale real-time RT-PCR

AU - Suzuki, Harukazu

AU - Okunishi, Rie

AU - Hashizume, Wataru

AU - Katayama, Shintaro

AU - Ninomiya, Noriko

AU - Osato, Naoki

AU - Sato, Kenjiro

AU - Nakamura, Mari

AU - Iida, Juri

AU - Kanamori, Mutsumi

AU - Hayashizaki, Yoshihide

N1 - Funding Information: We thank Dr. K. Polland (UCSC) for providing the HOPACH program and A. Hasegawa for technical assistance. We also thank the members of the Laboratory for Genome Exploration Research Group. This study was supported by MEXT Research Grants for the RIKEN Genome Exploration Research Project, for Advanced and Innovational Research Program in Life Science and for National Project on Genome Network Analysis to Y.H.

PY - 2004/8/27

Y1 - 2004/8/27

N2 - We established a medium-scale real-time RT-PCR system focusing on transcription factors and applied it to their expression profiles in the adult mouse 11 brain regions (http://genome.gsc.riken.jp/qRT-PCR/). Almost 90% of the examined genes showed significant expression in at least one region. We successfully extracted 179 region-specific genes by clustering analysis. Interestingly, the transcription factors involved in the development of the pituitary were still expressed in the adult pituitary, suggesting that they also play important roles in maintenance of the pituitary. These results provide unique molecular markers that may account for the molecular basis of the unique functions of specific brain regions.

AB - We established a medium-scale real-time RT-PCR system focusing on transcription factors and applied it to their expression profiles in the adult mouse 11 brain regions (http://genome.gsc.riken.jp/qRT-PCR/). Almost 90% of the examined genes showed significant expression in at least one region. We successfully extracted 179 region-specific genes by clustering analysis. Interestingly, the transcription factors involved in the development of the pituitary were still expressed in the adult pituitary, suggesting that they also play important roles in maintenance of the pituitary. These results provide unique molecular markers that may account for the molecular basis of the unique functions of specific brain regions.

KW - Real-time RT-PCR

KW - Region-specific

KW - Transcription factor

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Identification of region-specific transcription factor genes in the adult mouse brain by medium-scale real-time RT-PCR

Abstract

Keywords

ASJC Scopus subject areas

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