TY - JOUR
T1 - Identification of the homozygously deleted region at chromosome 1p36.2 in human neuroblastoma
AU - Nakagawara, Akira
AU - Ohira, Miki
AU - Kageyama, Hajime
AU - Mihara, Motohiro
AU - Furuta, Shigeyuki
AU - Machida, Taiichi
AU - Takayasu, Hajime
AU - Islam, Ashraful
AU - Nakamura, Yohko
AU - Takahashi, Masato
AU - Shishikura, Tomotane
AU - Kaneko, Yasuhiko
AU - Toyoda, Atsushi
AU - Hattori, Masahira
AU - Sakaki, Yoshiyuki
AU - Ohki, Misao
AU - Horii, Akira
AU - Soeda, Eiichi
AU - Inazawa, Johji
AU - Seki, Naohiko
AU - Kuma, Hidekazu
AU - Nozawa, Iwao
AU - Sakiyama, Shigeru
PY - 2000
Y1 - 2000
N2 - Background. We have identified for the first time a homozygously deleted region within the smallest region of overlap at 1p36.2-3 in two neuroblastoma cell lines. Procedure. The 800kb PAC contig covering the entire homozygously deleted region was made and sequenced. To date, approximately 70% of sequencing has been accomplished, and the estimated length of the deleted region was 500 kb. Results. Currently, we have found six genes within the region, which include three known genes as well as three other genes that have been reported during processing of our present project for the last 3 1/2 years. We report here the results of expression and mutation analyses of those genes. Conclusions. Full sequencing for the region of homozygous deletion as well as further analyses of the genes mapped within the region may reveal whether or not there is a neuroblastoma suppressor gene as proposed by the Knudson's two-hit hypothesis. (C) 2000 Wiley-Liss, Inc.
AB - Background. We have identified for the first time a homozygously deleted region within the smallest region of overlap at 1p36.2-3 in two neuroblastoma cell lines. Procedure. The 800kb PAC contig covering the entire homozygously deleted region was made and sequenced. To date, approximately 70% of sequencing has been accomplished, and the estimated length of the deleted region was 500 kb. Results. Currently, we have found six genes within the region, which include three known genes as well as three other genes that have been reported during processing of our present project for the last 3 1/2 years. We report here the results of expression and mutation analyses of those genes. Conclusions. Full sequencing for the region of homozygous deletion as well as further analyses of the genes mapped within the region may reveal whether or not there is a neuroblastoma suppressor gene as proposed by the Knudson's two-hit hypothesis. (C) 2000 Wiley-Liss, Inc.
KW - 1p36
KW - Homozygous deletion
KW - Neuroblastoma
KW - Tumor suppressor gene
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U2 - 10.1002/1096-911X(20001201)35:6<516::AID-MPO3>3.0.CO;2-Z
DO - 10.1002/1096-911X(20001201)35:6<516::AID-MPO3>3.0.CO;2-Z
M3 - Article
C2 - 11107106
AN - SCOPUS:0033661597
SN - 1545-5009
VL - 35
SP - 516
EP - 521
JO - Medical and Pediatric Oncology
JF - Medical and Pediatric Oncology
IS - 6
ER -
