TY - JOUR
T1 - Identification of the set of genes, including nonannotated morA, under the direct control of ModE in Escherichia coli
AU - Kurata, Tatsuaki
AU - Katayama, Akira
AU - Hiramatsu, Masakazu
AU - Kiguchi, Yuya
AU - Takeuchi, Masamitsu
AU - Watanabe, Tomoyuki
AU - Ogasawara, Hiroshi
AU - Ishihama, Akira
AU - Yamamotoa, Kaneyoshi
PY - 2013
Y1 - 2013
N2 - ModE is the molybdate-sensing transcription regulator that controls the expression of genes related to molybdate homeostasis in Escherichia coli. ModE is activated by binding molybdate and acts as both an activator and a repressor. By genomic systematic evolution of ligands by exponential enrichment (SELEX) screening and promoter reporter assays, we have identified a total of nine operons, including the hitherto identified modA, moaA, dmsA, and napF operons, of which six were activated by ModE and three were repressed. In addition, two promoters were newly identified and direct transcription of novel genes, referred to as morA and morB, located on antisense strands of yghW and torY, respectively. The morA gene encodes a short peptide, MorA, with an unusual initiation codon. Surprisingly, overexpression of the morA 5= untranslated region exhibited an inhibitory influence on colony formation of E. coli K-12.
AB - ModE is the molybdate-sensing transcription regulator that controls the expression of genes related to molybdate homeostasis in Escherichia coli. ModE is activated by binding molybdate and acts as both an activator and a repressor. By genomic systematic evolution of ligands by exponential enrichment (SELEX) screening and promoter reporter assays, we have identified a total of nine operons, including the hitherto identified modA, moaA, dmsA, and napF operons, of which six were activated by ModE and three were repressed. In addition, two promoters were newly identified and direct transcription of novel genes, referred to as morA and morB, located on antisense strands of yghW and torY, respectively. The morA gene encodes a short peptide, MorA, with an unusual initiation codon. Surprisingly, overexpression of the morA 5= untranslated region exhibited an inhibitory influence on colony formation of E. coli K-12.
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U2 - 10.1128/JB.00304-13
DO - 10.1128/JB.00304-13
M3 - Article
C2 - 23913318
AN - SCOPUS:84885457541
SN - 0021-9193
VL - 195
SP - 4496
EP - 4505
JO - Journal of Bacteriology
JF - Journal of Bacteriology
IS - 19
ER -