Abstract
We have developed a novel Immunoassay method using bacterial magnetic particles for the determination of immunoglobulin G (IgG). Fluorescein isothiocyanate (FITC) conjugated anti IgG-bacterial magnetic particles were prepared. The fluorescence quenching caused by agglutination of FITC-anti IgG antibody-bacterial magnetic particle conjugates was measured by using a fluorescence spectrophotometer. The aggregates based on specific immunoreaction were separated by a gelatin solution. The aggregation of bacterial magnetic particle conjugates was enhanced by application of a magnetic field. The relative fluorescence intensity correlated linearly with a concentration of IgG in the range 0.5-100 ng/mL.
| Original language | English |
|---|---|
| Pages (from-to) | 268-272 |
| Number of pages | 5 |
| Journal | Analytical Chemistry |
| Volume | 63 |
| Issue number | 3 |
| DOIs | |
| Publication status | Published - 1991 Feb 1 |
| Externally published | Yes |
ASJC Scopus subject areas
- Analytical Chemistry