Improvement of denitrifying reaction rate by enhanced substrate transport within a cell-immobilized space by electrophoresis

Satoshi Tsuneda*, Shuichi Kaku, Hiroshi Hayashi, Satoshi Ohgushi, Akihiko Terada, Akira Hirata

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

1 Citation (Scopus)


In order to improve the microbial reaction rate within a cell-immobilized space, this study experimentally investigated enhanced substrate transport by electrophoresis of a specific ion. Batch-mode and continuous feeding denitrification tests were carried out in an autotrophic denitrifying bioreactor using sulfur-oxidizing microbes as a model system. Seed sludge and powdered sulfur were immobilized on fibrous carrier medium, and an electric field was applied through the cell-immobilized zone via inert electrodes on both sides of vessel. In batch-mode denitrification tests, nitrogen removal rate reached 1.99 g-N·m-3·h-1 when the electric field of 18V was reversed at intervals of 30min, as compared with 0.40g-N·m -3·h-1 when the electric field was not applied. These findings demonstrated that the denitrification rate was increased by promoting the transport of NO3- ions across the cell-immobilized zone by electrophoresis. In addition, no reduction of NO 3- to NO2- occurred, indicating that decrease of nitrogen compound was due to biological reaction. Mathematical simulation analysis using a model involving Monod-type reaction kinetics and the Stokes formula for NO3- mobility was in general agreement with the experimental results. The results of the continuous feeding test also indicated that electrophoresis improved the nitrogen removal rate.

Original languageEnglish
Pages (from-to)507-513
Number of pages7
Journalkagaku kogaku ronbunshu
Issue number6
Publication statusPublished - 2006


  • Bacterial cell immobilization
  • Biological wastewater treatment
  • Denitrification
  • Electrophoresis
  • Fibrous slag

ASJC Scopus subject areas

  • General Chemistry
  • General Chemical Engineering


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