Improvement of oxygen carrying ability of hemoglobin vesicles by H 2O 2 elimination system

Tomoyasu Atoji; Motonari Aihara; Shinji Takeoka; Eishun Tsuchida

Improvement of oxygen carrying ability of hemoglobin vesicles by H ₂O ₂ elimination system

Tomoyasu Atoji^*, Motonari Aihara, Shinji Takeoka, Eishun Tsuchida

^*Corresponding author for this work

School of Advanced Science and Engineering

Research output: Contribution to conference › Paper › peer-review

Abstract

Oxyhemoglobin (oxyHb) of Hb-based oxygen carriers reacts with hydrogen peroxide (H ₂O ₂) to convert metHb and loses its oxygen binding ability. We have developed Hb-vesicles which encapsulate concentrated Hb with phospholipids bilayer membrane. Here, we constructed a suppression system of metHb formation within Hb vesicles using the peroxidase activity of metHb with L-Tyr. We could suppress the metHb formation of the oxyHb in the Hb vesicles by coencapsulating metHb and L-Tyr on the experiment of stepwise addition of H ₂O ₂ and autoxidation condition. Furthermore, this system was also effective in vivo. We concluded that H ₂O ₂ was effectively eliminated by the metHb/L-Tyr system in the Hb vesicles, and oxygen carrying ability was successfully prolonged.

Original language	English
Pages	4927-4928
Number of pages	2
Publication status	Published - 2005 Dec 1
Event	54th SPSJ Symposium on Macromolecules - Yamagata, Japan Duration: 2005 Sept 20 → 2005 Sept 22

Other

Other	54th SPSJ Symposium on Macromolecules
Country/Territory	Japan
City	Yamagata
Period	05/9/20 → 05/9/22

Keywords

Hb vesicles
Hydrogen peroxide/
L-tyrosine
MetHb
Peroxidase activity

ASJC Scopus subject areas

Engineering(all)

Cite this

@conference{8b0b471633814783945981a691a0b9b5,

title = "Improvement of oxygen carrying ability of hemoglobin vesicles by H 2O 2 elimination system",

abstract = "Oxyhemoglobin (oxyHb) of Hb-based oxygen carriers reacts with hydrogen peroxide (H 2O 2) to convert metHb and loses its oxygen binding ability. We have developed Hb-vesicles which encapsulate concentrated Hb with phospholipids bilayer membrane. Here, we constructed a suppression system of metHb formation within Hb vesicles using the peroxidase activity of metHb with L-Tyr. We could suppress the metHb formation of the oxyHb in the Hb vesicles by coencapsulating metHb and L-Tyr on the experiment of stepwise addition of H 2O 2 and autoxidation condition. Furthermore, this system was also effective in vivo. We concluded that H 2O 2 was effectively eliminated by the metHb/L-Tyr system in the Hb vesicles, and oxygen carrying ability was successfully prolonged.",

keywords = "Hb vesicles, Hydrogen peroxide/, L-tyrosine, MetHb, Peroxidase activity",

author = "Tomoyasu Atoji and Motonari Aihara and Shinji Takeoka and Eishun Tsuchida",

year = "2005",

month = dec,

day = "1",

language = "English",

pages = "4927--4928",

note = "54th SPSJ Symposium on Macromolecules ; Conference date: 20-09-2005 Through 22-09-2005",

}

TY - CONF

T1 - Improvement of oxygen carrying ability of hemoglobin vesicles by H 2O 2 elimination system

AU - Atoji, Tomoyasu

AU - Aihara, Motonari

AU - Takeoka, Shinji

AU - Tsuchida, Eishun

PY - 2005/12/1

Y1 - 2005/12/1

N2 - Oxyhemoglobin (oxyHb) of Hb-based oxygen carriers reacts with hydrogen peroxide (H 2O 2) to convert metHb and loses its oxygen binding ability. We have developed Hb-vesicles which encapsulate concentrated Hb with phospholipids bilayer membrane. Here, we constructed a suppression system of metHb formation within Hb vesicles using the peroxidase activity of metHb with L-Tyr. We could suppress the metHb formation of the oxyHb in the Hb vesicles by coencapsulating metHb and L-Tyr on the experiment of stepwise addition of H 2O 2 and autoxidation condition. Furthermore, this system was also effective in vivo. We concluded that H 2O 2 was effectively eliminated by the metHb/L-Tyr system in the Hb vesicles, and oxygen carrying ability was successfully prolonged.

AB - Oxyhemoglobin (oxyHb) of Hb-based oxygen carriers reacts with hydrogen peroxide (H 2O 2) to convert metHb and loses its oxygen binding ability. We have developed Hb-vesicles which encapsulate concentrated Hb with phospholipids bilayer membrane. Here, we constructed a suppression system of metHb formation within Hb vesicles using the peroxidase activity of metHb with L-Tyr. We could suppress the metHb formation of the oxyHb in the Hb vesicles by coencapsulating metHb and L-Tyr on the experiment of stepwise addition of H 2O 2 and autoxidation condition. Furthermore, this system was also effective in vivo. We concluded that H 2O 2 was effectively eliminated by the metHb/L-Tyr system in the Hb vesicles, and oxygen carrying ability was successfully prolonged.

KW - Hb vesicles

KW - Hydrogen peroxide/

KW - L-tyrosine

KW - MetHb

KW - Peroxidase activity

UR - http://www.scopus.com/inward/record.url?scp=33645560689&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33645560689&partnerID=8YFLogxK

M3 - Paper

AN - SCOPUS:33645560689

SP - 4927

EP - 4928

T2 - 54th SPSJ Symposium on Macromolecules

Y2 - 20 September 2005 through 22 September 2005

ER -