Improvement of transcutaneous fluorescent images with a depth-dependent point-spread function

Koichi Shimizu*, Koji Tochio, Yuji Kato

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

45 Citations (Scopus)


The point-spread function (PSF) for transcutaneous fluorescent imaging was obtained as an analytical solution in a closed form. It is applicable to cases in which the optical property of the image-blurring turbid medium is considered to be fairly homogeneous. We proposed a technique to improve a transcutaneous image by using depth-dependent PSF. Contrast of the fluorescent image was improved for depths of 1-15 mm in a scattering medium (μs′ = 1/mm). The visible depth was more than doubled with this technique. An experiment with a rat demonstrated considerable improvement of a transcutaneous image of the cerebral vein at a specified depth. The spread image of the heart was reduced to the correct size by use of the PSF with the actual depth of the heart.

Original languageEnglish
Pages (from-to)2154-2161
Number of pages8
JournalApplied Optics
Issue number11
Publication statusPublished - 2005 Apr 10
Externally publishedYes

ASJC Scopus subject areas

  • Atomic and Molecular Physics, and Optics
  • Engineering (miscellaneous)
  • Electrical and Electronic Engineering


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