In silico inference of inclusion membrane protein family in obligate intracellular parasites chlamydiae

Hidehiro Toh, Koshiro Miura, Mutsunori Shirai*, Masahira Hattori

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

41 Citations (Scopus)


Chlamydiae are obligate intracellular pathogens that proliferate only inside a vacuole, called an inclusion. Chlamydial Inc proteins are known to be a major component of the inclusion membrane, but little is known about the gene number and function. The Inc proteins share very low sequence similarity but a similar hydropathy profile among them. Using the hydropathy profile, we computationally searched the open reading frames (ORFs) having a similar profile and predicted 90 and 36 ORFs (Inc-like ORFs) as candidates for Inc proteins in Chlamydia pneumoniae J138 and Chlamydia trachomatis serovar D, respectively. On the other hand, only a few Inc-like ORFs were found in organisms other than chlamydiae, suggesting that the Inc-like ORFs are specific to chlamydiae. Comparative genome analysis also revealed that the Inc-like ORFs have multiplied and diverged as paralogues and orthologues in the chlamydial genomes, and that some Inc-like ORFs lacked the N-terminal portion or encoded the split form. The data suggest that these gene products constitute a large protein family and may play an important role in chlamydial infection, growth and survival in the host cell.

Original languageEnglish
Pages (from-to)9-17
Number of pages9
JournalDNA Research
Issue number1
Publication statusPublished - 2003
Externally publishedYes


  • Chlamydiae
  • Hydropathy profile
  • Inclusion membrane protein
  • Pathogen
  • Phase variation

ASJC Scopus subject areas

  • Genetics
  • Molecular Biology


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