In situ telomerase activity in pleural effusions: A promising marker for malignancy

Annika Dejmek; Naoyuki Yahata; Kazuma Ohyashiki; Yoshiro Ebihara; Masatoshi Kakihana; Takashi Hirano; Norihiko Kawate; Harubumi Kato

doi:10.1002/1097-0339(200101)24:1<11::AID-DC1001>3.0.CO;2-A

In situ telomerase activity in pleural effusions: A promising marker for malignancy

Annika Dejmek^*, Naoyuki Yahata, Kazuma Ohyashiki, Yoshiro Ebihara, Masatoshi Kakihana, Takashi Hirano, Norihiko Kawate, Harubumi Kato

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

26 Citations (Scopus)

Abstract

Telomerase activity in 16 pleural effusions was studied using an in situ telomerase repeat amplification protocol (TRAP) assay on cytospin preparations. Six of nine cytologically malignant specimens contained telomerase-positive cells (67%), and in two further specimens, suspicious positive cells were seen. Two of four atypical specimens contained telomerase-positive cells, whereas two benign cases were telomerase-negative. No mesothelial cells showed telomerase reactivity. Thus, telomerase activity was specific for malignancy and it was always found only in malignant cells. The results suggest that telomerase activity measured with this in situ method can be a valuable complement in the assessment of malignancy in pleural effusions. Diagn. Cytopathol. 2001;24: 11-15.

Original language	English
Pages (from-to)	11-15
Number of pages	5
Journal	Diagnostic cytopathology
Volume	24
Issue number	1
DOIs	https://doi.org/10.1002/1097-0339(200101)24:1<11::AID-DC1001>3.0.CO;2-A
Publication status	Published - 2001 Jan 16
Externally published	Yes

Keywords

Cytology
Effusions
TRAP in situ
Telomerase

ASJC Scopus subject areas

Pathology and Forensic Medicine
Histology

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10.1002/1097-0339(200101)24:1<11::AID-DC1001>3.0.CO;2-A

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@article{8b321b48b6d14afa8d33312e7f057136,

title = "In situ telomerase activity in pleural effusions: A promising marker for malignancy",

abstract = "Telomerase activity in 16 pleural effusions was studied using an in situ telomerase repeat amplification protocol (TRAP) assay on cytospin preparations. Six of nine cytologically malignant specimens contained telomerase-positive cells (67%), and in two further specimens, suspicious positive cells were seen. Two of four atypical specimens contained telomerase-positive cells, whereas two benign cases were telomerase-negative. No mesothelial cells showed telomerase reactivity. Thus, telomerase activity was specific for malignancy and it was always found only in malignant cells. The results suggest that telomerase activity measured with this in situ method can be a valuable complement in the assessment of malignancy in pleural effusions. Diagn. Cytopathol. 2001;24: 11-15.",

keywords = "Cytology, Effusions, TRAP in situ, Telomerase",

author = "Annika Dejmek and Naoyuki Yahata and Kazuma Ohyashiki and Yoshiro Ebihara and Masatoshi Kakihana and Takashi Hirano and Norihiko Kawate and Harubumi Kato",

year = "2001",

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doi = "10.1002/1097-0339(200101)24:1<11::AID-DC1001>3.0.CO;2-A",

language = "English",

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TY - JOUR

T1 - In situ telomerase activity in pleural effusions

T2 - A promising marker for malignancy

AU - Dejmek, Annika

AU - Yahata, Naoyuki

AU - Ohyashiki, Kazuma

AU - Ebihara, Yoshiro

AU - Kakihana, Masatoshi

AU - Hirano, Takashi

AU - Kawate, Norihiko

AU - Kato, Harubumi

PY - 2001/1/16

Y1 - 2001/1/16

N2 - Telomerase activity in 16 pleural effusions was studied using an in situ telomerase repeat amplification protocol (TRAP) assay on cytospin preparations. Six of nine cytologically malignant specimens contained telomerase-positive cells (67%), and in two further specimens, suspicious positive cells were seen. Two of four atypical specimens contained telomerase-positive cells, whereas two benign cases were telomerase-negative. No mesothelial cells showed telomerase reactivity. Thus, telomerase activity was specific for malignancy and it was always found only in malignant cells. The results suggest that telomerase activity measured with this in situ method can be a valuable complement in the assessment of malignancy in pleural effusions. Diagn. Cytopathol. 2001;24: 11-15.

AB - Telomerase activity in 16 pleural effusions was studied using an in situ telomerase repeat amplification protocol (TRAP) assay on cytospin preparations. Six of nine cytologically malignant specimens contained telomerase-positive cells (67%), and in two further specimens, suspicious positive cells were seen. Two of four atypical specimens contained telomerase-positive cells, whereas two benign cases were telomerase-negative. No mesothelial cells showed telomerase reactivity. Thus, telomerase activity was specific for malignancy and it was always found only in malignant cells. The results suggest that telomerase activity measured with this in situ method can be a valuable complement in the assessment of malignancy in pleural effusions. Diagn. Cytopathol. 2001;24: 11-15.

KW - Cytology

KW - Effusions

KW - TRAP in situ

KW - Telomerase

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U2 - 10.1002/1097-0339(200101)24:1<11::AID-DC1001>3.0.CO;2-A

DO - 10.1002/1097-0339(200101)24:1<11::AID-DC1001>3.0.CO;2-A

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ER -

In situ telomerase activity in pleural effusions: A promising marker for malignancy

Abstract

Keywords

ASJC Scopus subject areas

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