In vitro and in vivo phase changes of the mouse circadian clock by oxidative stress

Yu Tahara, Aya Yokota, Takuya Shiraishi, Shunya Yamada, Atsushi Haraguchi, Ayako Shinozaki, Shigenobu Shibata*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

12 Citations (Scopus)


Mammalian circadian rhythms are governed by an endogenous circadian clock system, including the molecular clock works in each cell and tissue. Adaptation of the circadian clock to different environmental stimuli such as light, food, and stress is essential for homeostasis maintenance. However, the influence of oxidative stress on the circadian clock phase is not fully understood in vitro and in vivo. Here, we examined the effects of hydrogen peroxide (H2O2)-induced oxidative stress on the PERIOD2::LUCIFERASE bioluminescence rhythm in mouse embryonic fibroblasts in vitro and in mouse peripheral tissues in vivo. The circadian clock phase changed with the dose of H2O2 and time of day in vitro; similar phase changes were observed in vivo in the circadian clocks of the peripheral tissues. In addition, mice treated with hemin-induced oxidative stress also showed phase changes of peripheral clocks, similarly as H2O2 treatment. Thus, oxidative stress can entrain circadian clock systems.

Original languageEnglish
Pages (from-to)1-7
Number of pages7
JournalJournal of Circadian Rhythms
Issue number1
Publication statusPublished - 2016
Externally publishedYes


  • Hydrogen peroxide
  • In vivo imaging
  • Kidney
  • Liver
  • Period2

ASJC Scopus subject areas

  • Physiology
  • Endocrine and Autonomic Systems


Dive into the research topics of 'In vitro and in vivo phase changes of the mouse circadian clock by oxidative stress'. Together they form a unique fingerprint.

Cite this