In vivo studies on methemoglobin formation by sodium nitrite

Kazuhiko Imaizumi*, Itiro Tyuma, Kiyohiro Imai, Hiroaki Kosaka, Yoshihiro Ueda

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

33 Citations (Scopus)


The oral LD5o of NaNO2 for rats was found to be 0.15 g per kg body weight. The methemoglobin level increased to 45-80%, 1 h after the administration of the LD5o dose and returned to the normal level after 24 h. The dose-maximum methemoglobin concentration curve was found to be Sshaped. Formation of nitrosyl hemoglobin preceded that of methemoglobin, its maximum concentration being a quarter of that of the latter derivative. In rats receiving 0.5% NaNO2 as drinking water, the concentration of methemoglobin showed a characteristic daily change (4 to 88%) due to the circadian rhythm of the animal in drinking. After 6 months, slight nitrosyl hemoglobin production, Heinz body formation, anisocytosis, and hypohemoglobinemia were observed.

Original languageEnglish
Pages (from-to)97-104
Number of pages8
JournalInternational Archives of Occupational and Environmental Health
Issue number2
Publication statusPublished - 1980 Jun
Externally publishedYes


  • Methemoglobin
  • Nitrite
  • Nitrosyl hemoglobin

ASJC Scopus subject areas

  • Public Health, Environmental and Occupational Health
  • Health, Toxicology and Mutagenesis


Dive into the research topics of 'In vivo studies on methemoglobin formation by sodium nitrite'. Together they form a unique fingerprint.

Cite this