Incorporation of DUF/FACT into chromatin enhances the accessibility of nucleosomal DNA

Hidetaka Seo; Koji Okuhara; Hitoshi Kurumizaka; Takatomi Yamada; Takehiko Shibata; Kunihiro Ohta; Tetsu Akiyama; Hiromu Murofushi

doi:10.1016/S0006-291X(03)00307-3

Incorporation of DUF/FACT into chromatin enhances the accessibility of nucleosomal DNA

Hidetaka Seo, Koji Okuhara, Hitoshi Kurumizaka, Takatomi Yamada, Takehiko Shibata, Kunihiro Ohta, Tetsu Akiyama, Hiromu Murofushi^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

6 Citations (Scopus)

Abstract

DNA unwinding factor (DUF) was discovered as an essential DNA replication factor in Xenopus egg extracts. DUF consists of an HMG protein and a homolog of Cdc68p/Spt16p, and has the capability of unwinding dsDNA. Here we have examined the interaction of DUF with chromatin. DUF was incorporated into chromatin assembled from sperm heads and from plasmid DNA in egg extracts. It was revealed that the chromatin assembled in egg extracts immunodepleted of DUF is less sensitive to micrococcal nuclease (NNase) digestion than that assembled in control extracts, indicating that chromatin containing DUF has more decompact structure than that without DUF. Also we found that DUF has a high affinity for core histones in vitro. We suggest that the function of DUF may be to make the chromatin structure accessible to replication factors.

Original language	English
Pages (from-to)	8-13
Number of pages	6
Journal	Biochemical and Biophysical Research Communications
Volume	303
Issue number	1
DOIs	https://doi.org/10.1016/S0006-291X(03)00307-3
Publication status	Published - 2003 Mar 28
Externally published	Yes

Keywords

Chromatin
CP complex
DNA replication
DNA unwinding factor
FACT
Xenopus egg

ASJC Scopus subject areas

Biochemistry
Biophysics
Molecular Biology

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10.1016/S0006-291X(03)00307-3

Cite this

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title = "Incorporation of DUF/FACT into chromatin enhances the accessibility of nucleosomal DNA",

abstract = "DNA unwinding factor (DUF) was discovered as an essential DNA replication factor in Xenopus egg extracts. DUF consists of an HMG protein and a homolog of Cdc68p/Spt16p, and has the capability of unwinding dsDNA. Here we have examined the interaction of DUF with chromatin. DUF was incorporated into chromatin assembled from sperm heads and from plasmid DNA in egg extracts. It was revealed that the chromatin assembled in egg extracts immunodepleted of DUF is less sensitive to micrococcal nuclease (NNase) digestion than that assembled in control extracts, indicating that chromatin containing DUF has more decompact structure than that without DUF. Also we found that DUF has a high affinity for core histones in vitro. We suggest that the function of DUF may be to make the chromatin structure accessible to replication factors.",

keywords = "Chromatin, CP complex, DNA replication, DNA unwinding factor, FACT, Xenopus egg",

author = "Hidetaka Seo and Koji Okuhara and Hitoshi Kurumizaka and Takatomi Yamada and Takehiko Shibata and Kunihiro Ohta and Tetsu Akiyama and Hiromu Murofushi",

year = "2003",

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doi = "10.1016/S0006-291X(03)00307-3",

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T1 - Incorporation of DUF/FACT into chromatin enhances the accessibility of nucleosomal DNA

AU - Seo, Hidetaka

AU - Okuhara, Koji

AU - Kurumizaka, Hitoshi

AU - Yamada, Takatomi

AU - Shibata, Takehiko

AU - Ohta, Kunihiro

AU - Akiyama, Tetsu

AU - Murofushi, Hiromu

PY - 2003/3/28

Y1 - 2003/3/28

N2 - DNA unwinding factor (DUF) was discovered as an essential DNA replication factor in Xenopus egg extracts. DUF consists of an HMG protein and a homolog of Cdc68p/Spt16p, and has the capability of unwinding dsDNA. Here we have examined the interaction of DUF with chromatin. DUF was incorporated into chromatin assembled from sperm heads and from plasmid DNA in egg extracts. It was revealed that the chromatin assembled in egg extracts immunodepleted of DUF is less sensitive to micrococcal nuclease (NNase) digestion than that assembled in control extracts, indicating that chromatin containing DUF has more decompact structure than that without DUF. Also we found that DUF has a high affinity for core histones in vitro. We suggest that the function of DUF may be to make the chromatin structure accessible to replication factors.

AB - DNA unwinding factor (DUF) was discovered as an essential DNA replication factor in Xenopus egg extracts. DUF consists of an HMG protein and a homolog of Cdc68p/Spt16p, and has the capability of unwinding dsDNA. Here we have examined the interaction of DUF with chromatin. DUF was incorporated into chromatin assembled from sperm heads and from plasmid DNA in egg extracts. It was revealed that the chromatin assembled in egg extracts immunodepleted of DUF is less sensitive to micrococcal nuclease (NNase) digestion than that assembled in control extracts, indicating that chromatin containing DUF has more decompact structure than that without DUF. Also we found that DUF has a high affinity for core histones in vitro. We suggest that the function of DUF may be to make the chromatin structure accessible to replication factors.

KW - Chromatin

KW - CP complex

KW - DNA replication

KW - DNA unwinding factor

KW - FACT

KW - Xenopus egg

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AN - SCOPUS:0037470795

SN - 0006-291X

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JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

IS - 1

ER -

Incorporation of DUF/FACT into chromatin enhances the accessibility of nucleosomal DNA

Abstract

Keywords

ASJC Scopus subject areas

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