Increase in the septal vasopressin content by prolyl endopeptidase inhibitors in rats

Naoyoshi Miura*, Shigenobu Shibata, Shigenori Watanabe

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

40 Citations (Scopus)


Prolyl endopeptidase (PEP; EC cleaves the Pro-Arg bond of arginine-vasopressin (AVP). This study investigated the effects of PEP inhibitors, 1-[1-(benzyloxycarbonyl)-l-prolyl]prolinal (Z-Pro-prolinal) and 1-[3-(2-indanylacetyl)-L-thioprolyl]pyrrolidine (Z-321), on the AVP content in the septum of rats. Oral administration of Z-Pro-prolinal (100 mg/kg) and Z-321 (100 mg/kg) significantly increased the septal AVP content. At 10 mg/kg, Z-321 slightly increased the content. In contrast, the d-thioprolyl form of Z-321 (100 mg/kg), which lacks an inhibitory effect on the enzyme, failed to affect the AVP content. These results indicate that PEP inhibitors increase the content of AVP through inhibition of the enzyme activity in vivo. Therefore, it is suggested that PEP may contribute to the degradation of endogenous AVP in the brain.

Original languageEnglish
Pages (from-to)128-130
Number of pages3
JournalNeuroscience Letters
Issue number1-2
Publication statusPublished - 1995 Aug 18
Externally publishedYes


  • Arginine-vasopressin
  • Inhibitors
  • Metabolism
  • Prolyl endopeptidase
  • Septum

ASJC Scopus subject areas

  • General Neuroscience


Dive into the research topics of 'Increase in the septal vasopressin content by prolyl endopeptidase inhibitors in rats'. Together they form a unique fingerprint.

Cite this