Increases in gene-targeting frequencies due to disruption of kueA as a ku80 homolog in citric acid-producing aspergillus niger

Yuki Honda; Keiichi Kobayashi; Kohtaro Kirimura

doi:10.1271/bbb.110015

Increases in gene-targeting frequencies due to disruption of kueA as a ku80 homolog in citric acid-producing aspergillus niger

Yuki Honda, Keiichi Kobayashi, Kohtaro Kirimura^*

^*Corresponding author for this work

School of Advanced Science and Engineering

Research output: Contribution to journal › Article › peer-review

12 Citations (Scopus)

Abstract

Low efficiencies of gene targeting hamper functional genomics in industrially important strains of Aspergillus niger. To generate strains showing high gene-targeting frequencies in A. niger WU-2223L producing citric acid, disruption of kueA encoding Ku80 homolog was performed. Disruption of kueA increased gene-targeting frequencies to 70%, and had no effect on citric acid production.

Original language	English
Pages (from-to)	1594-1596
Number of pages	3
Journal	Bioscience, Biotechnology and Biochemistry
Volume	75
Issue number	8
DOIs	https://doi.org/10.1271/bbb.110015
Publication status	Published - 2011

Keywords

Aspergillus niger
Citric acid production
Functional genomics
Gene-targeting
Ku80

ASJC Scopus subject areas

Biotechnology
Analytical Chemistry
Biochemistry
Applied Microbiology and Biotechnology
Molecular Biology
Organic Chemistry

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title = "Increases in gene-targeting frequencies due to disruption of kueA as a ku80 homolog in citric acid-producing aspergillus niger",

abstract = "Low efficiencies of gene targeting hamper functional genomics in industrially important strains of Aspergillus niger. To generate strains showing high gene-targeting frequencies in A. niger WU-2223L producing citric acid, disruption of kueA encoding Ku80 homolog was performed. Disruption of kueA increased gene-targeting frequencies to 70%, and had no effect on citric acid production.",

keywords = "Aspergillus niger, Citric acid production, Functional genomics, Gene-targeting, Ku80",

author = "Yuki Honda and Keiichi Kobayashi and Kohtaro Kirimura",

note = "Funding Information: This work was partially supported by Grants-in-Aid for Scientific Research (22580093), and by the Global COE program {\textquoteleft}{\textquoteleft}Center for Practical Chemical Wisdom{\textquoteright}{\textquoteright} by MEXT, Japan.",

year = "2011",

doi = "10.1271/bbb.110015",

language = "English",

volume = "75",

pages = "1594--1596",

journal = "Bioscience, Biotechnology and Biochemistry",

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publisher = "Japan Society for Bioscience Biotechnology and Agrochemistry",

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T1 - Increases in gene-targeting frequencies due to disruption of kueA as a ku80 homolog in citric acid-producing aspergillus niger

AU - Honda, Yuki

AU - Kobayashi, Keiichi

AU - Kirimura, Kohtaro

N1 - Funding Information: This work was partially supported by Grants-in-Aid for Scientific Research (22580093), and by the Global COE program ‘‘Center for Practical Chemical Wisdom’’ by MEXT, Japan.

PY - 2011

Y1 - 2011

N2 - Low efficiencies of gene targeting hamper functional genomics in industrially important strains of Aspergillus niger. To generate strains showing high gene-targeting frequencies in A. niger WU-2223L producing citric acid, disruption of kueA encoding Ku80 homolog was performed. Disruption of kueA increased gene-targeting frequencies to 70%, and had no effect on citric acid production.

AB - Low efficiencies of gene targeting hamper functional genomics in industrially important strains of Aspergillus niger. To generate strains showing high gene-targeting frequencies in A. niger WU-2223L producing citric acid, disruption of kueA encoding Ku80 homolog was performed. Disruption of kueA increased gene-targeting frequencies to 70%, and had no effect on citric acid production.

KW - Aspergillus niger

KW - Citric acid production

KW - Functional genomics

KW - Gene-targeting

KW - Ku80

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SN - 0916-8451

VL - 75

SP - 1594

EP - 1596

JO - Bioscience, Biotechnology and Biochemistry

JF - Bioscience, Biotechnology and Biochemistry

IS - 8

ER -

Increases in gene-targeting frequencies due to disruption of kueA as a ku80 homolog in citric acid-producing aspergillus niger

Abstract

Keywords

ASJC Scopus subject areas

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