Intense exercise causes decrease in expression of both endothelial NO synthase and tissue NOx level in hearts

Motoyuki Iemitsu; Takashi Miyauchi; Seiji Maeda; Koichi Yuki; Tsutomu Kobayashi; Yoshito Kumagai; Nobuhiro Shimojo; Iwao Yamaguchi; Mitsuo Matsuda

doi:10.1152/ajpregu.2000.279.3.r951

Intense exercise causes decrease in expression of both endothelial NO synthase and tissue NOx level in hearts

Motoyuki Iemitsu, Takashi Miyauchi^*, Seiji Maeda, Koichi Yuki, Tsutomu Kobayashi, Yoshito Kumagai, Nobuhiro Shimojo, Iwao Yamaguchi, Mitsuo Matsuda

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

48 Citations (Scopus)

Abstract

Cardiac myocytes produce nitric oxide (NO). We studied the effects of intense exercise on the expression of NO synthase (NOS) and the tissue level of nitrite (NO₂/^-)/nitrate (NO₃/^-) (i.e., NOx), which are stable end products of NO in the heart. Rats ran on a treadmill for 45 min. Immediately after this exercise, the heart was quickly removed. Control rats remained at rest during the same 45-min period. The mRNA level of endothelial NOS (eNOS) in the heart was markedly lower in the exercised rats than in the control rats. Western blot analysis confirmed downregulation of eNOS protein in the heart after exercise. Tissue NOx level in the heart was significantly lower in the exercised rats than in the control rats. The present study revealed for the first time that production of NO in the heart is decreased by intense exercise. Because NO attenuates positive inotropic and chronotropic responses to β₁-adrenergic stimulation in the heart, the decrease in cardiac production of NO by intense exercise may contribute to the acceleration of increase in myocardial contractility and heart rate during intense exercise.

Original language	English
Pages (from-to)	R951-R959
Journal	American Journal of Physiology - Regulatory Integrative and Comparative Physiology
Volume	279
Issue number	3 48-3
DOIs	https://doi.org/10.1152/ajpregu.2000.279.3.r951
Publication status	Published - 2000
Externally published	Yes

Keywords

Chronotropic effect
Inotropic effect
Nitric oxide
Nitric oxide synthase
Treadmill running

ASJC Scopus subject areas

Medicine(all)

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10.1152/ajpregu.2000.279.3.r951

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Iemitsu, M., Miyauchi, T., Maeda, S., Yuki, K., Kobayashi, T., Kumagai, Y., Shimojo, N., Yamaguchi, I., & Matsuda, M. (2000). Intense exercise causes decrease in expression of both endothelial NO synthase and tissue NOx level in hearts. American Journal of Physiology - Regulatory Integrative and Comparative Physiology, 279(3 48-3), R951-R959. https://doi.org/10.1152/ajpregu.2000.279.3.r951

Iemitsu, M, Miyauchi, T, Maeda, S, Yuki, K, Kobayashi, T, Kumagai, Y, Shimojo, N, Yamaguchi, I & Matsuda, M 2000, 'Intense exercise causes decrease in expression of both endothelial NO synthase and tissue NOx level in hearts', American Journal of Physiology - Regulatory Integrative and Comparative Physiology, vol. 279, no. 3 48-3, pp. R951-R959. https://doi.org/10.1152/ajpregu.2000.279.3.r951

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abstract = "Cardiac myocytes produce nitric oxide (NO). We studied the effects of intense exercise on the expression of NO synthase (NOS) and the tissue level of nitrite (NO2/-)/nitrate (NO3/-) (i.e., NOx), which are stable end products of NO in the heart. Rats ran on a treadmill for 45 min. Immediately after this exercise, the heart was quickly removed. Control rats remained at rest during the same 45-min period. The mRNA level of endothelial NOS (eNOS) in the heart was markedly lower in the exercised rats than in the control rats. Western blot analysis confirmed downregulation of eNOS protein in the heart after exercise. Tissue NOx level in the heart was significantly lower in the exercised rats than in the control rats. The present study revealed for the first time that production of NO in the heart is decreased by intense exercise. Because NO attenuates positive inotropic and chronotropic responses to β1-adrenergic stimulation in the heart, the decrease in cardiac production of NO by intense exercise may contribute to the acceleration of increase in myocardial contractility and heart rate during intense exercise.",

keywords = "Chronotropic effect, Inotropic effect, Nitric oxide, Nitric oxide synthase, Treadmill running",

author = "Motoyuki Iemitsu and Takashi Miyauchi and Seiji Maeda and Koichi Yuki and Tsutomu Kobayashi and Yoshito Kumagai and Nobuhiro Shimojo and Iwao Yamaguchi and Mitsuo Matsuda",

year = "2000",

doi = "10.1152/ajpregu.2000.279.3.r951",

language = "English",

volume = "279",

pages = "R951--R959",

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T1 - Intense exercise causes decrease in expression of both endothelial NO synthase and tissue NOx level in hearts

AU - Iemitsu, Motoyuki

AU - Miyauchi, Takashi

AU - Maeda, Seiji

AU - Yuki, Koichi

AU - Kobayashi, Tsutomu

AU - Kumagai, Yoshito

AU - Shimojo, Nobuhiro

AU - Yamaguchi, Iwao

AU - Matsuda, Mitsuo

PY - 2000

Y1 - 2000

N2 - Cardiac myocytes produce nitric oxide (NO). We studied the effects of intense exercise on the expression of NO synthase (NOS) and the tissue level of nitrite (NO2/-)/nitrate (NO3/-) (i.e., NOx), which are stable end products of NO in the heart. Rats ran on a treadmill for 45 min. Immediately after this exercise, the heart was quickly removed. Control rats remained at rest during the same 45-min period. The mRNA level of endothelial NOS (eNOS) in the heart was markedly lower in the exercised rats than in the control rats. Western blot analysis confirmed downregulation of eNOS protein in the heart after exercise. Tissue NOx level in the heart was significantly lower in the exercised rats than in the control rats. The present study revealed for the first time that production of NO in the heart is decreased by intense exercise. Because NO attenuates positive inotropic and chronotropic responses to β1-adrenergic stimulation in the heart, the decrease in cardiac production of NO by intense exercise may contribute to the acceleration of increase in myocardial contractility and heart rate during intense exercise.

AB - Cardiac myocytes produce nitric oxide (NO). We studied the effects of intense exercise on the expression of NO synthase (NOS) and the tissue level of nitrite (NO2/-)/nitrate (NO3/-) (i.e., NOx), which are stable end products of NO in the heart. Rats ran on a treadmill for 45 min. Immediately after this exercise, the heart was quickly removed. Control rats remained at rest during the same 45-min period. The mRNA level of endothelial NOS (eNOS) in the heart was markedly lower in the exercised rats than in the control rats. Western blot analysis confirmed downregulation of eNOS protein in the heart after exercise. Tissue NOx level in the heart was significantly lower in the exercised rats than in the control rats. The present study revealed for the first time that production of NO in the heart is decreased by intense exercise. Because NO attenuates positive inotropic and chronotropic responses to β1-adrenergic stimulation in the heart, the decrease in cardiac production of NO by intense exercise may contribute to the acceleration of increase in myocardial contractility and heart rate during intense exercise.

KW - Chronotropic effect

KW - Inotropic effect

KW - Nitric oxide

KW - Nitric oxide synthase

KW - Treadmill running

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Intense exercise causes decrease in expression of both endothelial NO synthase and tissue NOx level in hearts

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