TY - JOUR
T1 - Interaction between phospholipid assemblies and hemoglobin (hb)
AU - Takeoka, Shinji
AU - Terase, Kouichiro
AU - Yokohama, Hiroaki
AU - Sakai, Hiromi
AU - Nishide, Hiroyuki
AU - Tsuchida, Eishun
N1 - Funding Information:
This work was partly supported by a Grant-in-Aid from the Ministry of Education, Science and Culture, Japan (No. 04750757), and the Cosmetology Research Foundation.
PY - 1994/1/1
Y1 - 1994/1/1
N2 - In order to prepare Hb-vesicles having a high oxygen-transporting ability, it is important to encapsulate concentrated Hb with a mixed lipid membrane having low lamellarity (n). The concentration of Hb in the Hb-vesicles ([Hb]in) increased with the Hb concentration used for the preparation of Hb-vesicles. Hb-vesicles with smaller lamellarity could be obtained by lowering the preparation temperature. The encapsulation of Hb was significantly influenced by the ionic strength and the solution pH because lipids and Hb are electrolytes. The lamellarity decreases with increasing solution pH because of electrostatic repulsion between the surfaces of lipid membranes. On the other hand, [Hb]in increases when the solution pH is lowered below the p/ of Hb (7.0 at 25°C) due to electrostatic interaction between Hb and the surface of the membrane. This interaction decreases with the ionic strength of the Hb solution, leading to a lower [Hb]in.
AB - In order to prepare Hb-vesicles having a high oxygen-transporting ability, it is important to encapsulate concentrated Hb with a mixed lipid membrane having low lamellarity (n). The concentration of Hb in the Hb-vesicles ([Hb]in) increased with the Hb concentration used for the preparation of Hb-vesicles. Hb-vesicles with smaller lamellarity could be obtained by lowering the preparation temperature. The encapsulation of Hb was significantly influenced by the ionic strength and the solution pH because lipids and Hb are electrolytes. The lamellarity decreases with increasing solution pH because of electrostatic repulsion between the surfaces of lipid membranes. On the other hand, [Hb]in increases when the solution pH is lowered below the p/ of Hb (7.0 at 25°C) due to electrostatic interaction between Hb and the surface of the membrane. This interaction decreases with the ionic strength of the Hb solution, leading to a lower [Hb]in.
UR - http://www.scopus.com/inward/record.url?scp=0028336437&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0028336437&partnerID=8YFLogxK
U2 - 10.1080/10601329409349720
DO - 10.1080/10601329409349720
M3 - Article
AN - SCOPUS:0028336437
SN - 1060-1325
VL - 31
SP - 97
EP - 108
JO - Journal of Macromolecular Science, Part A
JF - Journal of Macromolecular Science, Part A
IS - 1
ER -