Interaction between phospholipid assemblies and hemoglobin (hb)

Shinji Takeoka, Kouichiro Terase, Hiroaki Yokohama, Hiromi Sakai, Hiroyuki Nishide, Eishun Tsuchida

Research output: Contribution to journalArticlepeer-review

13 Citations (Scopus)


In order to prepare Hb-vesicles having a high oxygen-transporting ability, it is important to encapsulate concentrated Hb with a mixed lipid membrane having low lamellarity (n). The concentration of Hb in the Hb-vesicles ([Hb]in) increased with the Hb concentration used for the preparation of Hb-vesicles. Hb-vesicles with smaller lamellarity could be obtained by lowering the preparation temperature. The encapsulation of Hb was significantly influenced by the ionic strength and the solution pH because lipids and Hb are electrolytes. The lamellarity decreases with increasing solution pH because of electrostatic repulsion between the surfaces of lipid membranes. On the other hand, [Hb]in increases when the solution pH is lowered below the p/ of Hb (7.0 at 25°C) due to electrostatic interaction between Hb and the surface of the membrane. This interaction decreases with the ionic strength of the Hb solution, leading to a lower [Hb]in.

Original languageEnglish
Pages (from-to)97-108
Number of pages12
JournalJournal of Macromolecular Science, Part A
Issue number1
Publication statusPublished - 1994 Jan 1

ASJC Scopus subject areas

  • Ceramics and Composites
  • Chemistry(all)
  • Polymers and Plastics
  • Materials Chemistry


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