TY - JOUR
T1 - Interaction of hemoglobin vesicles, a cellular-type artificial oxygen carrier, with human plasma
T2 - Effects on coagulation, kallikrein-kinin, and complement systems
AU - Abe, Hideki
AU - Fujihara, Mitsuhiro
AU - Azuma, Hiroshi
AU - Ikeda, Hisami
AU - Ikebuchi, Kenji
AU - Takeoka, Shinji
AU - Tsuchida, Eishun
AU - Harashima, Hideyoshi
N1 - Funding Information:
Laboratory for Molecular Design of Pharmaceutics and COE Program in the 21st Century, Graduate School of Pharmaceutical Sciences, Hokkaido University, Sapporo, Japan Abstract: Hemoglobin vesicles (HbVs), cellular-type artificial oxygen carriers containing human hemoglobin, were assessed for their biocompatibility by mixing with human plasma in vitro. Among three kinds of HbVs (PEG-DPEA-HbV, PEG-DPPG-HbV and DPPG-HbV), PEG-DPEA-HbV did not affect the extrinsic or intrinsic coagulation activities of the plasma, while PEG-DPPG-HbV and DPPG-HbV tended to shorten the intrinsic coagulation time. The kallikrein-kinin cascade of the plasma was slightly activated by PEG-DPPG-HbV This study was supported, in part, by a Health Science Research Grant (Artificial Blood Project) from the Ministry of Health and Welfare, Japan, and by Grants-in-Aid for Scientific Research from the Japanese Ministry of Education, Science, Sports and Culture.
PY - 2006/4/1
Y1 - 2006/4/1
N2 - Hemoglobin vesicles (HbVs), cellular-type artificial oxygen carriers containing human hemoglobin, were assessed for their biocompatibility by mixing with human plasma in vitro. Among three kinds of HbVs (PEG-DPEA-HbV, PEG-DPPG-HbV and DPPG-HbV), PEG-DPEA-HbV did not affect the extrinsic or intrinsic coagulation activities of the plasma, while PEG-DPPG-HbV and DPPG-HbV tended to shorten the intrinsic coagulation time. The kallikrein-kinin cascade of the plasma was slightly activated by PEG-DPPG-HbV and DPPG-HbV, but not by PEG-DPEA-HbV. The complement consumption of the plasma was observed by incubation with DPPG-HbV, but not with PEG-DPEA-HbV or PEG-DPPG-HbV. These results indicate that PEG-DPEA-HbV has a higher biocompatibility with human plasma.
AB - Hemoglobin vesicles (HbVs), cellular-type artificial oxygen carriers containing human hemoglobin, were assessed for their biocompatibility by mixing with human plasma in vitro. Among three kinds of HbVs (PEG-DPEA-HbV, PEG-DPPG-HbV and DPPG-HbV), PEG-DPEA-HbV did not affect the extrinsic or intrinsic coagulation activities of the plasma, while PEG-DPPG-HbV and DPPG-HbV tended to shorten the intrinsic coagulation time. The kallikrein-kinin cascade of the plasma was slightly activated by PEG-DPPG-HbV and DPPG-HbV, but not by PEG-DPEA-HbV. The complement consumption of the plasma was observed by incubation with DPPG-HbV, but not with PEG-DPEA-HbV or PEG-DPPG-HbV. These results indicate that PEG-DPEA-HbV has a higher biocompatibility with human plasma.
KW - Complement
KW - Hemoglobin vesicles
KW - Kallikrein-Kinin
KW - Liposome
KW - Oxygen carrier
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U2 - 10.1080/00207450500428204
DO - 10.1080/00207450500428204
M3 - Article
C2 - 16519400
AN - SCOPUS:31144450037
SN - 1073-1199
VL - 34
SP - 1
EP - 10
JO - Artificial Cells, Blood Substitutes, and Biotechnology
JF - Artificial Cells, Blood Substitutes, and Biotechnology
IS - 1
ER -