TY - JOUR
T1 - Interference with cell-to-cell movement of Tomato mosaic virus by transient overexpression of Arabidopsis thaliana KELP homologs from different plant species
AU - Sasaki, Nobumitsu
AU - Odawara, Tatsuro
AU - Deguchi, Masakazu
AU - Matsushita, Yasuhiko
AU - Nyunoya, Hiroshi
N1 - Funding Information:
Acknowledgments We thank Yuko Takahashi for technical assistance and Takuya Ogata for critical reading of the manuscript. This work was supported in part by a Grant-in-Aid for Scientific Research (to H.N., nos. 15028206 and 17580038), a Grant-in-Aid for Scientific Research (C) (to Y.M., nos. 15580031) and a Grant-in-Aid for Young Scientists (B) (to N.S., no. 20780030) from the Ministry of Education, Culture, Sports, Science, and Technology of Japan and a Grant-in-Aid for Young Scientists (start-up) (to N.S., no. 18880010) from Japan Society for the Promotion of Science.
PY - 2010/1
Y1 - 2010/1
N2 - The KELP protein of Arabidopsis thaliana (AtKELP), which binds to the Tomato mosaic virus (ToMV) movement protein in vitro, can interfere with cell-to-cell movement of the virus under transient overexpression conditions. In this study, we constructed expression plasmids encoding a fluorescent protein fused to AtKELP or to its homolog from Brassica, Nicotiana, Solanum, or Oryza species. When expressed transiently, all the AtKELP homologs inhibited the cell-to-cell movement of ToMV with different efficiencies. The results of domain swapping between two selected AtKELP homologs suggest the importance of combination between the N- and C-terminal half regions for high inhibitory function.
AB - The KELP protein of Arabidopsis thaliana (AtKELP), which binds to the Tomato mosaic virus (ToMV) movement protein in vitro, can interfere with cell-to-cell movement of the virus under transient overexpression conditions. In this study, we constructed expression plasmids encoding a fluorescent protein fused to AtKELP or to its homolog from Brassica, Nicotiana, Solanum, or Oryza species. When expressed transiently, all the AtKELP homologs inhibited the cell-to-cell movement of ToMV with different efficiencies. The results of domain swapping between two selected AtKELP homologs suggest the importance of combination between the N- and C-terminal half regions for high inhibitory function.
KW - Cell-to-cell movement
KW - Inhibitory factor
KW - KELP
KW - Movement protein
KW - Tomato mosaic virus
KW - Transient overexpression
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U2 - 10.1007/s10327-009-0214-9
DO - 10.1007/s10327-009-0214-9
M3 - Article
AN - SCOPUS:75549083947
SN - 1345-2630
VL - 76
SP - 69
EP - 73
JO - Journal of General Plant Pathology
JF - Journal of General Plant Pathology
IS - 1
ER -