Interference with initial and short-distance cell-to-cell movement of Tomato mosaic virus in transgenic tobacco plants with high expression of BcKELP, a virus movement protein interactor from Brassica campestris

Nobumitsu Sasaki; Tatsuro Odawara; Shoko Nagai; Kazuma Yoshimura; Yasuhiko Matsushita; Hiroshi Nyunoya

doi:10.1016/j.pmpp.2012.01.003

Interference with initial and short-distance cell-to-cell movement of Tomato mosaic virus in transgenic tobacco plants with high expression of BcKELP, a virus movement protein interactor from Brassica campestris

Nobumitsu Sasaki^*, Tatsuro Odawara, Shoko Nagai, Kazuma Yoshimura, Yasuhiko Matsushita, Hiroshi Nyunoya

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

5 Citations (Scopus)

Abstract

A putative transcriptional coactivator KELP of . Arabidopsis thaliana and its homolog of . Brassica campestris (BcKELP) can bind the movement protein (MP) of . Tomato mosaic virus (ToMV) . in vitro and, when transiently over-expressed, interfere with the cell-to-cell movement of ToMV. In this study, we generated and selected transgenic . Nicotiana tabacum lines expressing BcKELP constitutively and examined their resistance to ToMV. We also investigated changes in MP localization in initially infected . Nicotiana benthamiana cells expressing BcKELP transiently. Our results indicate that a high expression of BcKELP can reduce viral initial and short-distance movement probably through disturbance of plasmodesmal targeting of MP.

Original language	English
Pages (from-to)	38-44
Number of pages	7
Journal	Physiological and Molecular Plant Pathology
Volume	78
DOIs	https://doi.org/10.1016/j.pmpp.2012.01.003
Publication status	Published - 2012 Apr
Externally published	Yes

Keywords

Cell-to-cell movement
Movement protein
Plant protection
Plasmodesmal targeting
Tomato mosaic virus
Transcriptional coactivator
Transgenic tobacco

ASJC Scopus subject areas

Genetics
Plant Science

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10.1016/j.pmpp.2012.01.003

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Sasaki, N., Odawara, T., Nagai, S., Yoshimura, K., Matsushita, Y., & Nyunoya, H. (2012). Interference with initial and short-distance cell-to-cell movement of Tomato mosaic virus in transgenic tobacco plants with high expression of BcKELP, a virus movement protein interactor from Brassica campestris. Physiological and Molecular Plant Pathology, 78, 38-44. https://doi.org/10.1016/j.pmpp.2012.01.003

Interference with initial and short-distance cell-to-cell movement of Tomato mosaic virus in transgenic tobacco plants with high expression of BcKELP, a virus movement protein interactor from Brassica campestris. / Sasaki, Nobumitsu; Odawara, Tatsuro; Nagai, Shoko et al.
In: Physiological and Molecular Plant Pathology, Vol. 78, 04.2012, p. 38-44.

Research output: Contribution to journal › Article › peer-review

Sasaki, N, Odawara, T, Nagai, S, Yoshimura, K, Matsushita, Y & Nyunoya, H 2012, 'Interference with initial and short-distance cell-to-cell movement of Tomato mosaic virus in transgenic tobacco plants with high expression of BcKELP, a virus movement protein interactor from Brassica campestris', Physiological and Molecular Plant Pathology, vol. 78, pp. 38-44. https://doi.org/10.1016/j.pmpp.2012.01.003

Sasaki N, Odawara T, Nagai S, Yoshimura K, Matsushita Y, Nyunoya H. Interference with initial and short-distance cell-to-cell movement of Tomato mosaic virus in transgenic tobacco plants with high expression of BcKELP, a virus movement protein interactor from Brassica campestris. Physiological and Molecular Plant Pathology. 2012 Apr;78:38-44. doi: 10.1016/j.pmpp.2012.01.003

@article{d0a0cc6cd5a845368c40fd525dfbae4e,

title = "Interference with initial and short-distance cell-to-cell movement of Tomato mosaic virus in transgenic tobacco plants with high expression of BcKELP, a virus movement protein interactor from Brassica campestris",

abstract = "A putative transcriptional coactivator KELP of . Arabidopsis thaliana and its homolog of . Brassica campestris (BcKELP) can bind the movement protein (MP) of . Tomato mosaic virus (ToMV) . in vitro and, when transiently over-expressed, interfere with the cell-to-cell movement of ToMV. In this study, we generated and selected transgenic . Nicotiana tabacum lines expressing BcKELP constitutively and examined their resistance to ToMV. We also investigated changes in MP localization in initially infected . Nicotiana benthamiana cells expressing BcKELP transiently. Our results indicate that a high expression of BcKELP can reduce viral initial and short-distance movement probably through disturbance of plasmodesmal targeting of MP.",

keywords = "Cell-to-cell movement, Movement protein, Plant protection, Plasmodesmal targeting, Tomato mosaic virus, Transcriptional coactivator, Transgenic tobacco",

author = "Nobumitsu Sasaki and Tatsuro Odawara and Shoko Nagai and Kazuma Yoshimura and Yasuhiko Matsushita and Hiroshi Nyunoya",

note = "Funding Information: We acknowledge Dr. Tetsuro Meshi and Dr. Yuichiro Watanabe for providing us with piL.G3 and pTocJ/GFP, respectively. We also thank Yuko Takahashi for technical assistance. This work was supported in part by a Grant-in-Aid for Scientific Research (to H.N., nos. 15028206 and 17580038 ), a Grant-in-Aid for Scientific Research (C) (to Y.M., no. 15580031 ), Grants-in-Aid for Encouragement of Scientists (B) (to Y.M., no. 13760035 , and to N.S., no. 20780030 ) from the Ministry of Education, Culture, Sports, Science, and Technology of Japan, and Grants-in-Aid for Encouragement of Scientists (A) (to Y.M., no. 11760033 ) and Young Scientists (start-up) (to N.S., no. 18880010 ) from Japan Society for the Promotion of Science.",

year = "2012",

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doi = "10.1016/j.pmpp.2012.01.003",

language = "English",

volume = "78",

pages = "38--44",

journal = "Physiological and Molecular Plant Pathology",

issn = "0885-5765",

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T1 - Interference with initial and short-distance cell-to-cell movement of Tomato mosaic virus in transgenic tobacco plants with high expression of BcKELP, a virus movement protein interactor from Brassica campestris

AU - Sasaki, Nobumitsu

AU - Odawara, Tatsuro

AU - Nagai, Shoko

AU - Yoshimura, Kazuma

AU - Matsushita, Yasuhiko

AU - Nyunoya, Hiroshi

N1 - Funding Information: We acknowledge Dr. Tetsuro Meshi and Dr. Yuichiro Watanabe for providing us with piL.G3 and pTocJ/GFP, respectively. We also thank Yuko Takahashi for technical assistance. This work was supported in part by a Grant-in-Aid for Scientific Research (to H.N., nos. 15028206 and 17580038 ), a Grant-in-Aid for Scientific Research (C) (to Y.M., no. 15580031 ), Grants-in-Aid for Encouragement of Scientists (B) (to Y.M., no. 13760035 , and to N.S., no. 20780030 ) from the Ministry of Education, Culture, Sports, Science, and Technology of Japan, and Grants-in-Aid for Encouragement of Scientists (A) (to Y.M., no. 11760033 ) and Young Scientists (start-up) (to N.S., no. 18880010 ) from Japan Society for the Promotion of Science.

PY - 2012/4

Y1 - 2012/4

N2 - A putative transcriptional coactivator KELP of . Arabidopsis thaliana and its homolog of . Brassica campestris (BcKELP) can bind the movement protein (MP) of . Tomato mosaic virus (ToMV) . in vitro and, when transiently over-expressed, interfere with the cell-to-cell movement of ToMV. In this study, we generated and selected transgenic . Nicotiana tabacum lines expressing BcKELP constitutively and examined their resistance to ToMV. We also investigated changes in MP localization in initially infected . Nicotiana benthamiana cells expressing BcKELP transiently. Our results indicate that a high expression of BcKELP can reduce viral initial and short-distance movement probably through disturbance of plasmodesmal targeting of MP.

AB - A putative transcriptional coactivator KELP of . Arabidopsis thaliana and its homolog of . Brassica campestris (BcKELP) can bind the movement protein (MP) of . Tomato mosaic virus (ToMV) . in vitro and, when transiently over-expressed, interfere with the cell-to-cell movement of ToMV. In this study, we generated and selected transgenic . Nicotiana tabacum lines expressing BcKELP constitutively and examined their resistance to ToMV. We also investigated changes in MP localization in initially infected . Nicotiana benthamiana cells expressing BcKELP transiently. Our results indicate that a high expression of BcKELP can reduce viral initial and short-distance movement probably through disturbance of plasmodesmal targeting of MP.

KW - Cell-to-cell movement

KW - Movement protein

KW - Plant protection

KW - Plasmodesmal targeting

KW - Tomato mosaic virus

KW - Transcriptional coactivator

KW - Transgenic tobacco

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SN - 0885-5765

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JO - Physiological and Molecular Plant Pathology

JF - Physiological and Molecular Plant Pathology

ER -

Interference with initial and short-distance cell-to-cell movement of Tomato mosaic virus in transgenic tobacco plants with high expression of BcKELP, a virus movement protein interactor from Brassica campestris

Abstract

Keywords

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