TY - JOUR
T1 - Interleukin-1β and interleukin-6 affect electrophysiological properties of thalamic relay cells
AU - Samios, Vinicius Nikolaos
AU - Inoue, Takafumi
N1 - Funding Information:
The authors thank to Drs. Mariko Miyata and Yuichi Takeuchi for technical discussions. This work was supported by a Project for Private Universities: matching fund subsidy from MEXT (Ministry of Education, Culture, Sports, Science and Technology) of Japan (T.I., S1201006) , Grants for Excellent Graduate Schools (Practical Chemical Wisdom, MEXT of Japan, V.N.S.) and Japanese Government (MEXT) scholarship (V.N.S.).
Publisher Copyright:
© 2014 Elsevier Ireland Ltd and the Japan Neuroscience Society.
PY - 2014
Y1 - 2014
N2 - By acknowledging the relation between brain and body in health and disease, inflammatory processes may play a key role in this reciprocal relation. Pro-inflammatory cytokines such as interleukin-1β (IL-1β) and interleukin-6 (IL-6) are some of the agents involved in those processes. What exactly is their role in the CNS however is not that clear so far. To address the question of how pro-inflammatory cytokines may affect information processing at the cellular and molecular levels, relay neurons in the thalamic dorsal lateral geniculate nucleus in mouse brain slices were exposed to those cytokines and studied with the patch-clamp technique. IL-1β promoted hyperpolarization of the resting membrane potential (Vrest), decrease of input resistance (Rin), decrease of Ih rectification, decrease in action potential (AP) threshold and decrease in the number of APs in low threshold calcium spike (LTS) bursts, while IL-6 promoted decrease of Rin and decrease in the number of APs in LTS bursts. Computer simulations provided candidates for ionic conductance affected by those cytokines. Collectively, these findings demonstrate that IL-1β and IL-6 have modulatory effects on electrophysiological properties of thalamic neurons, implying that the thalamic functions may be affected by systemic disorders that present with high levels of those cytokines.
AB - By acknowledging the relation between brain and body in health and disease, inflammatory processes may play a key role in this reciprocal relation. Pro-inflammatory cytokines such as interleukin-1β (IL-1β) and interleukin-6 (IL-6) are some of the agents involved in those processes. What exactly is their role in the CNS however is not that clear so far. To address the question of how pro-inflammatory cytokines may affect information processing at the cellular and molecular levels, relay neurons in the thalamic dorsal lateral geniculate nucleus in mouse brain slices were exposed to those cytokines and studied with the patch-clamp technique. IL-1β promoted hyperpolarization of the resting membrane potential (Vrest), decrease of input resistance (Rin), decrease of Ih rectification, decrease in action potential (AP) threshold and decrease in the number of APs in low threshold calcium spike (LTS) bursts, while IL-6 promoted decrease of Rin and decrease in the number of APs in LTS bursts. Computer simulations provided candidates for ionic conductance affected by those cytokines. Collectively, these findings demonstrate that IL-1β and IL-6 have modulatory effects on electrophysiological properties of thalamic neurons, implying that the thalamic functions may be affected by systemic disorders that present with high levels of those cytokines.
KW - IL-1β
KW - IL-6
KW - Interleukin
KW - Patch-clamp
KW - Thalamic relay cell
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U2 - 10.1016/j.neures.2014.06.011
DO - 10.1016/j.neures.2014.06.011
M3 - Article
C2 - 25091392
AN - SCOPUS:84927570716
SN - 0168-0102
VL - 87
SP - 16
EP - 25
JO - Neuroscience Research
JF - Neuroscience Research
IS - C
ER -