Intermolecular interaction of actin revealed by a dynamic light scattering technique

Noriko Kanzaki; Taro Q.P. Uyeda; Kazuo Onuma

doi:10.1021/jp054865g

Intermolecular interaction of actin revealed by a dynamic light scattering technique

Noriko Kanzaki^*, Taro Q.P. Uyeda, Kazuo Onuma

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

20 Citations (Scopus)

Abstract

The intermolecular interaction force of actin was studied by a dynamic light scattering technique. The mutual diffusion coefficients (D) of monomeric actin were accurately determined in a G-buffer with a low concentration of KCl from 0 to 10 mM. The translational diffusion coefficient was obtained as D ₀ = (87 ± 3) × 10^-12 m²· s^-1 at 25°C and pH 7.4, which gives a hydrodynamic radius of monomeric actin of r_H = 2.8 ± 0.1 nm. The Derjaguin-Landau- Verwey-Overbeek (DLVO) theory, assuming electrostatic and van der Waals potentials, failed to describe the change in interaction parameter (A) with KCl concentration, but the extended DLVO theory succeeded if an additional repulsive potential was assumed. The Hamaker constant of actin in the Ca²⁺-ATP bound state was determined for the first time as AH = 10.4 ± 0.6 k _BT.

Original language	English
Pages (from-to)	2881-2887
Number of pages	7
Journal	Journal of Physical Chemistry B
Volume	110
Issue number	6
DOIs	https://doi.org/10.1021/jp054865g
Publication status	Published - 2006 Feb 16
Externally published	Yes

ASJC Scopus subject areas

Physical and Theoretical Chemistry
Surfaces, Coatings and Films
Materials Chemistry

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title = "Intermolecular interaction of actin revealed by a dynamic light scattering technique",

abstract = "The intermolecular interaction force of actin was studied by a dynamic light scattering technique. The mutual diffusion coefficients (D) of monomeric actin were accurately determined in a G-buffer with a low concentration of KCl from 0 to 10 mM. The translational diffusion coefficient was obtained as D 0 = (87 ± 3) × 10-12 m2· s-1 at 25°C and pH 7.4, which gives a hydrodynamic radius of monomeric actin of rH = 2.8 ± 0.1 nm. The Derjaguin-Landau- Verwey-Overbeek (DLVO) theory, assuming electrostatic and van der Waals potentials, failed to describe the change in interaction parameter (A) with KCl concentration, but the extended DLVO theory succeeded if an additional repulsive potential was assumed. The Hamaker constant of actin in the Ca2+-ATP bound state was determined for the first time as AH = 10.4 ± 0.6 k BT.",

author = "Noriko Kanzaki and Uyeda, {Taro Q.P.} and Kazuo Onuma",

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journal = "Journal of Physical Chemistry B",

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T1 - Intermolecular interaction of actin revealed by a dynamic light scattering technique

AU - Kanzaki, Noriko

AU - Uyeda, Taro Q.P.

AU - Onuma, Kazuo

PY - 2006/2/16

Y1 - 2006/2/16

N2 - The intermolecular interaction force of actin was studied by a dynamic light scattering technique. The mutual diffusion coefficients (D) of monomeric actin were accurately determined in a G-buffer with a low concentration of KCl from 0 to 10 mM. The translational diffusion coefficient was obtained as D 0 = (87 ± 3) × 10-12 m2· s-1 at 25°C and pH 7.4, which gives a hydrodynamic radius of monomeric actin of rH = 2.8 ± 0.1 nm. The Derjaguin-Landau- Verwey-Overbeek (DLVO) theory, assuming electrostatic and van der Waals potentials, failed to describe the change in interaction parameter (A) with KCl concentration, but the extended DLVO theory succeeded if an additional repulsive potential was assumed. The Hamaker constant of actin in the Ca2+-ATP bound state was determined for the first time as AH = 10.4 ± 0.6 k BT.

AB - The intermolecular interaction force of actin was studied by a dynamic light scattering technique. The mutual diffusion coefficients (D) of monomeric actin were accurately determined in a G-buffer with a low concentration of KCl from 0 to 10 mM. The translational diffusion coefficient was obtained as D 0 = (87 ± 3) × 10-12 m2· s-1 at 25°C and pH 7.4, which gives a hydrodynamic radius of monomeric actin of rH = 2.8 ± 0.1 nm. The Derjaguin-Landau- Verwey-Overbeek (DLVO) theory, assuming electrostatic and van der Waals potentials, failed to describe the change in interaction parameter (A) with KCl concentration, but the extended DLVO theory succeeded if an additional repulsive potential was assumed. The Hamaker constant of actin in the Ca2+-ATP bound state was determined for the first time as AH = 10.4 ± 0.6 k BT.

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JF - Journal of Physical Chemistry B

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Intermolecular interaction of actin revealed by a dynamic light scattering technique

Abstract

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