Intermolecular interaction of actin revealed by a dynamic light scattering technique

Noriko Kanzaki*, Taro Q.P. Uyeda, Kazuo Onuma

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

20 Citations (Scopus)


The intermolecular interaction force of actin was studied by a dynamic light scattering technique. The mutual diffusion coefficients (D) of monomeric actin were accurately determined in a G-buffer with a low concentration of KCl from 0 to 10 mM. The translational diffusion coefficient was obtained as D 0 = (87 ± 3) × 10-12 m2· s-1 at 25°C and pH 7.4, which gives a hydrodynamic radius of monomeric actin of rH = 2.8 ± 0.1 nm. The Derjaguin-Landau- Verwey-Overbeek (DLVO) theory, assuming electrostatic and van der Waals potentials, failed to describe the change in interaction parameter (A) with KCl concentration, but the extended DLVO theory succeeded if an additional repulsive potential was assumed. The Hamaker constant of actin in the Ca2+-ATP bound state was determined for the first time as AH = 10.4 ± 0.6 k BT.

Original languageEnglish
Pages (from-to)2881-2887
Number of pages7
JournalJournal of Physical Chemistry B
Issue number6
Publication statusPublished - 2006 Feb 16
Externally publishedYes

ASJC Scopus subject areas

  • Physical and Theoretical Chemistry
  • Surfaces, Coatings and Films
  • Materials Chemistry


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