Internal motion of F-actin in 10-6-10-3s time range studied by transient absorption anisotropy: Detection of torsional motion

Koshin Mihashi; Hideyuki Yoshimura; Takuhiro Nishio; Akira Ikegami; Kazuhiko Kinosita

Internal motion of F-actin in 10^-6-10^-3s time range studied by transient absorption anisotropy: Detection of torsional motion

Koshin Mihashi^*, Hideyuki Yoshimura, Takuhiro Nishio, Akira Ikegami, Kazuhiko Kinosita

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

15 Citations (Scopus)

Abstract

By means of laser flash photolysis, the transient absorption anisotropy (TAA) of the triplet probe, 5-iodoacetamide-Eosin, labeling rabbit skeletal F-actin was measured in the 10^-6-10^-3 s time range. The TAA curve at 20°C showed a relatively slow decay phase covering several hundred microseconds and a large residual anisotropy (∼0.1 at 2 ms). After analysis with Barkley & Zimm's formula, it was concluded that the TAA of Eosin-F-actin can be approximated by the anisotropy decay due to torsional motion of F-actin.

Original language	English
Pages (from-to)	1705-1707
Number of pages	3
Journal	Journal of Biochemistry
Volume	93
Issue number	6
Publication status	Published - 1983
Externally published	Yes

ASJC Scopus subject areas

Statistics, Probability and Uncertainty
Applied Mathematics
Physiology (medical)
Radiology Nuclear Medicine and imaging
Molecular Biology
Biochemistry

Cite this

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title = "Internal motion of F-actin in 10-6-10-3s time range studied by transient absorption anisotropy: Detection of torsional motion",

abstract = "By means of laser flash photolysis, the transient absorption anisotropy (TAA) of the triplet probe, 5-iodoacetamide-Eosin, labeling rabbit skeletal F-actin was measured in the 10-6-10-3 s time range. The TAA curve at 20°C showed a relatively slow decay phase covering several hundred microseconds and a large residual anisotropy (∼0.1 at 2 ms). After analysis with Barkley & Zimm's formula, it was concluded that the TAA of Eosin-F-actin can be approximated by the anisotropy decay due to torsional motion of F-actin.",

author = "Koshin Mihashi and Hideyuki Yoshimura and Takuhiro Nishio and Akira Ikegami and Kazuhiko Kinosita",

year = "1983",

language = "English",

volume = "93",

pages = "1705--1707",

journal = "Journal of Biochemistry",

issn = "0021-924X",

publisher = "Oxford University Press",

number = "6",

}

TY - JOUR

T1 - Internal motion of F-actin in 10-6-10-3s time range studied by transient absorption anisotropy

T2 - Detection of torsional motion

AU - Mihashi, Koshin

AU - Yoshimura, Hideyuki

AU - Nishio, Takuhiro

AU - Ikegami, Akira

AU - Kinosita, Kazuhiko

PY - 1983

Y1 - 1983

N2 - By means of laser flash photolysis, the transient absorption anisotropy (TAA) of the triplet probe, 5-iodoacetamide-Eosin, labeling rabbit skeletal F-actin was measured in the 10-6-10-3 s time range. The TAA curve at 20°C showed a relatively slow decay phase covering several hundred microseconds and a large residual anisotropy (∼0.1 at 2 ms). After analysis with Barkley & Zimm's formula, it was concluded that the TAA of Eosin-F-actin can be approximated by the anisotropy decay due to torsional motion of F-actin.

AB - By means of laser flash photolysis, the transient absorption anisotropy (TAA) of the triplet probe, 5-iodoacetamide-Eosin, labeling rabbit skeletal F-actin was measured in the 10-6-10-3 s time range. The TAA curve at 20°C showed a relatively slow decay phase covering several hundred microseconds and a large residual anisotropy (∼0.1 at 2 ms). After analysis with Barkley & Zimm's formula, it was concluded that the TAA of Eosin-F-actin can be approximated by the anisotropy decay due to torsional motion of F-actin.

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M3 - Article

C2 - 6885746

AN - SCOPUS:0020773301

SN - 0021-924X

VL - 93

SP - 1705

EP - 1707

JO - Journal of Biochemistry

JF - Journal of Biochemistry

IS - 6

ER -

Internal motion of F-actin in 10^-6-10^-3s time range studied by transient absorption anisotropy: Detection of torsional motion

Abstract

ASJC Scopus subject areas

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