Interplay between folding/unfolding and helix/coil transitions in giant DNA

Sergey V. Mikhailenko, Vladimir G. Sergeyev*, Anatoly A. Zinchenko, Marat O. Gallyamov, Igor V. Yaminsky, Kenichi Yoshikawa

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

19 Citations (Scopus)


It has been well established that double-stranded DNA undergoes a melting, or helix/coil, transition into a single-stranded coil state with an increase in temperature. On the other hand, it has recently been found that, at a fixed temperature, long DNA, larger than several kilobase pairs, exhibits a discrete transition, or switching, between elongated and folded states, preserving its double-stranded structure, with the addition of various condensation agents, such as alcohol, hydrophilic polymer, multivalent cation, and cationic surfactant. In the present study, we examined the interplay between the folding/unfolding transition and the helix/coil transition in individual giant DNA molecules, by observing the conformation of single molecular chains with fluorescence microscopy. The results indicate that the helix-to-coil transition tightly cooperates with the unfolding transition in DNA.

Original languageEnglish
Pages (from-to)597-603
Number of pages7
Issue number4
Publication statusPublished - 2000
Externally publishedYes

ASJC Scopus subject areas

  • Bioengineering
  • Biomaterials
  • Polymers and Plastics
  • Materials Chemistry


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