Intracellular click reaction with a fluorescent chemical Ca2+ indicator to prolong its cytosolic retention

Yoshiaki Takei; Atsushi Murata; Kento Yamagishi; Satoshi Arai; Hideki Nakamura; Takafumi Inoue; Shinji Takeoka

doi:10.1039/c3cc42489h

Intracellular click reaction with a fluorescent chemical Ca²⁺ indicator to prolong its cytosolic retention

Yoshiaki Takei, Atsushi Murata, Kento Yamagishi, Satoshi Arai, Hideki Nakamura, Takafumi Inoue, Shinji Takeoka^*

^*Corresponding author for this work

School of Advanced Science and Engineering

Research output: Contribution to journal › Article › peer-review

8 Citations (Scopus)

Abstract

The powerful strategy of “intracellular click reaction” was used to retain a chemical Ca²⁺ indicator in the cytosol. Specifically, a novel clickable Ca²⁺ indicator “N₃-fura-2 AM” was coupled with dibenzylcyclooctyl-modified biomacromolecules via copper-free click reaction in living cells and Ca²⁺ oscillation was observed for an extended period of time.

Original language	English
Pages (from-to)	7313-7315
Number of pages	3
Journal	Chemical Communications
Volume	49
Issue number	66
DOIs	https://doi.org/10.1039/c3cc42489h
Publication status	Published - 2013 Jul 23

ASJC Scopus subject areas

Catalysis
Electronic, Optical and Magnetic Materials
Ceramics and Composites
General Chemistry
Surfaces, Coatings and Films
Metals and Alloys
Materials Chemistry

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abstract = "The powerful strategy of “intracellular click reaction” was used to retain a chemical Ca2+ indicator in the cytosol. Specifically, a novel clickable Ca2+ indicator “N3-fura-2 AM” was coupled with dibenzylcyclooctyl-modified biomacromolecules via copper-free click reaction in living cells and Ca2+ oscillation was observed for an extended period of time.",

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AU - Takei, Yoshiaki

AU - Murata, Atsushi

AU - Yamagishi, Kento

AU - Arai, Satoshi

AU - Nakamura, Hideki

AU - Inoue, Takafumi

AU - Takeoka, Shinji

PY - 2013/7/23

Y1 - 2013/7/23

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AB - The powerful strategy of “intracellular click reaction” was used to retain a chemical Ca2+ indicator in the cytosol. Specifically, a novel clickable Ca2+ indicator “N3-fura-2 AM” was coupled with dibenzylcyclooctyl-modified biomacromolecules via copper-free click reaction in living cells and Ca2+ oscillation was observed for an extended period of time.

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Intracellular click reaction with a fluorescent chemical Ca²⁺ indicator to prolong its cytosolic retention

Abstract

ASJC Scopus subject areas

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