Investigation on the mechanical properties of contracted collagen gels as a scaffold for tissue engineering

Zhonggang Feng*, M. Yamato, T. Akutsu, T. Nakamura, T. Okano, M. Umezu

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

92 Citations (Scopus)


In this article the mechanical properties of contracted collagen gels were investigated thoroughly by means of uniaxial tensile test. Large type I collagen-Dulbecco's Modified Eagle Medium (DMEM) gels (each was 26 ml in volume, 1.67 mg/ml collagen concentration), each populated with about 2.5 × 106 human fibroblasts, were made in 100 mm diameter plastic dishes precoated with albumin for floating the gels in DMEM. Such identically treated gels were divided into three groups for the mechanical measurements at different culture periods (2, 4, and 10 weeks). Rapid contraction occurred within the first 3 days and then the contraction went slowly in the rest period until it reached about 13% of its original size. The stress-strain curve of the contracted collagen gels demonstrated an exponential behavior at low stress region, followed by linear region, a point of yielding, and finally an ultimate stress point at which the maximum stress was reached. The mechanical strength increased in the first few weeks and then decreased as the culture went on. It is obvious that the collagen fibrils formed and were forced to orientate to the tensile direction after the test. The stress relaxation and cyclic creep phenomena were observed. Based on the morphological analysis of transmission electron microscopy (TEM) of the gels, a nonlinear visco-elastic-plastic constitutive formula was proposed, which was able to reproduce the rheological phenomena of the gels. This experiment shows that the human fibroblasts significantly contracted collagen gels so as to achieve certain mechanical strength, which makes it possible to be a scaffold for tissue engineering. However, a further method to reinforce the mechanical strength by several folds must be considered. Meanwhile, the rheological phenomena should be taken into account in the fabrication and application of the structure.

Original languageEnglish
Pages (from-to)84-91
Number of pages8
JournalArtificial Organs
Issue number1
Publication statusPublished - 2003
Externally publishedYes


  • Collagen type I
  • Contracted collagen gel
  • Human fibroblast
  • Mechanical properties
  • Rheological properties
  • Tissue engineering

ASJC Scopus subject areas

  • Bioengineering
  • Medicine (miscellaneous)
  • Biomaterials
  • Biomedical Engineering


Dive into the research topics of 'Investigation on the mechanical properties of contracted collagen gels as a scaffold for tissue engineering'. Together they form a unique fingerprint.

Cite this