Isolation and characterization of a GnRH-like peptide from Octopus vulgaris

Eiko Iwakoshi, Kyoko Takuwa-Kuroda, Yuko Fujisawa, Miki Hisada, Kazuyoshi Ukena, Kazuyoshi Tsutsui, Kazuyoshi Tsutsui*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

163 Citations (Scopus)


Gonadotropin-releasing hormone (GnRH) is the key peptide in the hypothalamo-hypophysial-gonadal axis, the core of regulation of reproduction in vertebrates. In this study, an octopus peptide with structural features similar to vertebrate GnRHs was isolated from brains of Octopus vulgaris. This peptide showed luteinizing hormone-releasing activity in quail anterior pituitary cells. A cDNA encoding the precursor protein was cloned. The RT-PCR transcripts were expressed in the supraesophageal and subesophageal brains, peduncle complex, and optic gland. The presence of the peptide in the different brain region was confirmed with enzyme-linked immunosorbent assay and time-of-flight mass spectrometric analysis. Immunoreactive neuronal cell bodies and fibers were observed in the subpedunculate lobe that controls the optic-gland activity. Optic gland nerves and glandular cells in the optic gland were immunostained. The isolated peptide may be octopus GnRH that contributes to octopus reproduction not only as a neurohormone but also as an endocrine hormone.

Original languageEnglish
Pages (from-to)1187-1193
Number of pages7
JournalBiochemical and Biophysical Research Communications
Issue number5
Publication statusPublished - 2002
Externally publishedYes


  • cDNA cloning
  • Immunohistochemistry
  • Octopus GnRH
  • RT-PCR Southern blot analysis
  • TOF-MS

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology


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