Isolation of Magnetospirillum magneticum AMB-1 mutants defective in bacterial magnetic particle synthesis by transposon mutagenesis

Aris Tri Wahyudi; Haruko Takeyama; Tadashi Matsunaga

doi:10.1385/ABAB:91-93:1-9:147

Isolation of Magnetospirillum magneticum AMB-1 mutants defective in bacterial magnetic particle synthesis by transposon mutagenesis

Aris Tri Wahyudi, Haruko Takeyama, Tadashi Matsunaga^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

23 Citations (Scopus)

Abstract

Nonmagnetic mutants of Magnetospirillum magneticum AMB-1 were recovered following mini-Tn5 transposon mutagenesis. Transconjugants with kanamycin resistance were obtained at a frequency of 2.7 × 10^-7 per recipient. Of 3327 transconjugants, 62 were defective for bacterial magnetic particle (BMP) synthesis. The frequency of independent transposition events for nonmagnetic mutants was about 1.4% in transconjugants. Further analysis of DNA sequences flanking transposon by inverted polymerase chain reaction allowed isolation of at least 10 genes or DNA sequences involved in BMP synthesis in M. magneticum AMB-1.

Original language	English
Pages (from-to)	147-154
Number of pages	8
Journal	Applied Biochemistry and Biotechnology - Part A Enzyme Engineering and Biotechnology
Volume	91-93
DOIs	https://doi.org/10.1385/ABAB:91-93:1-9:147
Publication status	Published - 2001
Externally published	Yes

Keywords

Bacterial magnetic particles
Inverse polymerase chain reaction
Magnetospirillum magneticum AMB-1
Mini-Tn5
Sequence analysis
Transposon mutagenesis

ASJC Scopus subject areas

Biotechnology
Bioengineering
Biochemistry
Applied Microbiology and Biotechnology
Molecular Biology

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10.1385/ABAB:91-93:1-9:147

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Isolation of Magnetospirillum magneticum AMB-1 mutants defective in bacterial magnetic particle synthesis by transposon mutagenesis. / Wahyudi, Aris Tri; Takeyama, Haruko; Matsunaga, Tadashi.
In: Applied Biochemistry and Biotechnology - Part A Enzyme Engineering and Biotechnology, Vol. 91-93, 2001, p. 147-154.

Research output: Contribution to journal › Article › peer-review

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title = "Isolation of Magnetospirillum magneticum AMB-1 mutants defective in bacterial magnetic particle synthesis by transposon mutagenesis",

abstract = "Nonmagnetic mutants of Magnetospirillum magneticum AMB-1 were recovered following mini-Tn5 transposon mutagenesis. Transconjugants with kanamycin resistance were obtained at a frequency of 2.7 × 10-7 per recipient. Of 3327 transconjugants, 62 were defective for bacterial magnetic particle (BMP) synthesis. The frequency of independent transposition events for nonmagnetic mutants was about 1.4% in transconjugants. Further analysis of DNA sequences flanking transposon by inverted polymerase chain reaction allowed isolation of at least 10 genes or DNA sequences involved in BMP synthesis in M. magneticum AMB-1.",

keywords = "Bacterial magnetic particles, Inverse polymerase chain reaction, Magnetospirillum magneticum AMB-1, Mini-Tn5, Sequence analysis, Transposon mutagenesis",

author = "Wahyudi, {Aris Tri} and Haruko Takeyama and Tadashi Matsunaga",

note = "Funding Information: This work was supported in part by Grant-in-Aid for Scientific Research on Priority Area (A) no. 10145102, The Ministry of Education, Science, Sport and Culture.",

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AU - Wahyudi, Aris Tri

AU - Takeyama, Haruko

AU - Matsunaga, Tadashi

N1 - Funding Information: This work was supported in part by Grant-in-Aid for Scientific Research on Priority Area (A) no. 10145102, The Ministry of Education, Science, Sport and Culture.

PY - 2001

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N2 - Nonmagnetic mutants of Magnetospirillum magneticum AMB-1 were recovered following mini-Tn5 transposon mutagenesis. Transconjugants with kanamycin resistance were obtained at a frequency of 2.7 × 10-7 per recipient. Of 3327 transconjugants, 62 were defective for bacterial magnetic particle (BMP) synthesis. The frequency of independent transposition events for nonmagnetic mutants was about 1.4% in transconjugants. Further analysis of DNA sequences flanking transposon by inverted polymerase chain reaction allowed isolation of at least 10 genes or DNA sequences involved in BMP synthesis in M. magneticum AMB-1.

AB - Nonmagnetic mutants of Magnetospirillum magneticum AMB-1 were recovered following mini-Tn5 transposon mutagenesis. Transconjugants with kanamycin resistance were obtained at a frequency of 2.7 × 10-7 per recipient. Of 3327 transconjugants, 62 were defective for bacterial magnetic particle (BMP) synthesis. The frequency of independent transposition events for nonmagnetic mutants was about 1.4% in transconjugants. Further analysis of DNA sequences flanking transposon by inverted polymerase chain reaction allowed isolation of at least 10 genes or DNA sequences involved in BMP synthesis in M. magneticum AMB-1.

KW - Bacterial magnetic particles

KW - Inverse polymerase chain reaction

KW - Magnetospirillum magneticum AMB-1

KW - Mini-Tn5

KW - Sequence analysis

KW - Transposon mutagenesis

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JO - Applied Biochemistry and Biotechnology - Part A Enzyme Engineering and Biotechnology

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Isolation of Magnetospirillum magneticum AMB-1 mutants defective in bacterial magnetic particle synthesis by transposon mutagenesis

Abstract

Keywords

ASJC Scopus subject areas

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