Isolation of Nitrospira belonging to sublineage II from a wastewater treatment plant

Norisuke Ushiki; Hirotsugu Fujitani; Yoshiteru Aoi; Satoshi Tsuneda

doi:10.1264/jsme2.ME13042

Isolation of Nitrospira belonging to sublineage II from a wastewater treatment plant

Norisuke Ushiki, Hirotsugu Fujitani, Yoshiteru Aoi, Satoshi Tsuneda^*

^*Corresponding author for this work

School of Advanced Science and Engineering

Research output: Contribution to journal › Article › peer-review

67 Citations (Scopus)

Abstract

Nitrite oxidation is a key step in nitrogen removal in biological wastewater treatment plants. Recently, two phylogenetically different Nitrospira (sublineages I and II) have been recognized as the numerically dominant nitriteoxidizing bacteria in wastewater treatment plants. However, Nitrospira sublineage II inhabiting activated sludge was not isolated and its detailed properties were unclear. In this study, we developed a new method for the isolation of Nitrospira forming micro-colonies using a cell sorter. We obtained a novel pure strain "Nitrospira japonica" from the activated sludge. Subsequently, phylogenetic and physiological analyses revealed that Nitrospira japonica belongs to sublineage II and grew in medium containing formate. This method has the potential to isolate other uncultured microorganisms forming micro-colonies.

Original language	English
Pages (from-to)	346-353
Number of pages	8
Journal	Microbes and Environments
Volume	28
Issue number	3
DOIs	https://doi.org/10.1264/jsme2.ME13042
Publication status	Published - 2013

Keywords

Cell sorter
Formate
Micro-colony
Nitrification
Uncultured bacteria

ASJC Scopus subject areas

Ecology, Evolution, Behavior and Systematics
Soil Science
Plant Science

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10.1264/jsme2.ME13042

Cite this

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abstract = "Nitrite oxidation is a key step in nitrogen removal in biological wastewater treatment plants. Recently, two phylogenetically different Nitrospira (sublineages I and II) have been recognized as the numerically dominant nitriteoxidizing bacteria in wastewater treatment plants. However, Nitrospira sublineage II inhabiting activated sludge was not isolated and its detailed properties were unclear. In this study, we developed a new method for the isolation of Nitrospira forming micro-colonies using a cell sorter. We obtained a novel pure strain {"}Nitrospira japonica{"} from the activated sludge. Subsequently, phylogenetic and physiological analyses revealed that Nitrospira japonica belongs to sublineage II and grew in medium containing formate. This method has the potential to isolate other uncultured microorganisms forming micro-colonies.",

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author = "Norisuke Ushiki and Hirotsugu Fujitani and Yoshiteru Aoi and Satoshi Tsuneda",

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T1 - Isolation of Nitrospira belonging to sublineage II from a wastewater treatment plant

AU - Ushiki, Norisuke

AU - Fujitani, Hirotsugu

AU - Aoi, Yoshiteru

AU - Tsuneda, Satoshi

PY - 2013

Y1 - 2013

N2 - Nitrite oxidation is a key step in nitrogen removal in biological wastewater treatment plants. Recently, two phylogenetically different Nitrospira (sublineages I and II) have been recognized as the numerically dominant nitriteoxidizing bacteria in wastewater treatment plants. However, Nitrospira sublineage II inhabiting activated sludge was not isolated and its detailed properties were unclear. In this study, we developed a new method for the isolation of Nitrospira forming micro-colonies using a cell sorter. We obtained a novel pure strain "Nitrospira japonica" from the activated sludge. Subsequently, phylogenetic and physiological analyses revealed that Nitrospira japonica belongs to sublineage II and grew in medium containing formate. This method has the potential to isolate other uncultured microorganisms forming micro-colonies.

AB - Nitrite oxidation is a key step in nitrogen removal in biological wastewater treatment plants. Recently, two phylogenetically different Nitrospira (sublineages I and II) have been recognized as the numerically dominant nitriteoxidizing bacteria in wastewater treatment plants. However, Nitrospira sublineage II inhabiting activated sludge was not isolated and its detailed properties were unclear. In this study, we developed a new method for the isolation of Nitrospira forming micro-colonies using a cell sorter. We obtained a novel pure strain "Nitrospira japonica" from the activated sludge. Subsequently, phylogenetic and physiological analyses revealed that Nitrospira japonica belongs to sublineage II and grew in medium containing formate. This method has the potential to isolate other uncultured microorganisms forming micro-colonies.

KW - Cell sorter

KW - Formate

KW - Micro-colony

KW - Nitrification

KW - Uncultured bacteria

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Isolation of Nitrospira belonging to sublineage II from a wastewater treatment plant

Abstract

Keywords

ASJC Scopus subject areas

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