Isolation of Nitrospira belonging to sublineage II from a wastewater treatment plant

Norisuke Ushiki, Hirotsugu Fujitani, Yoshiteru Aoi, Satoshi Tsuneda*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

67 Citations (Scopus)


Nitrite oxidation is a key step in nitrogen removal in biological wastewater treatment plants. Recently, two phylogenetically different Nitrospira (sublineages I and II) have been recognized as the numerically dominant nitriteoxidizing bacteria in wastewater treatment plants. However, Nitrospira sublineage II inhabiting activated sludge was not isolated and its detailed properties were unclear. In this study, we developed a new method for the isolation of Nitrospira forming micro-colonies using a cell sorter. We obtained a novel pure strain "Nitrospira japonica" from the activated sludge. Subsequently, phylogenetic and physiological analyses revealed that Nitrospira japonica belongs to sublineage II and grew in medium containing formate. This method has the potential to isolate other uncultured microorganisms forming micro-colonies.

Original languageEnglish
Pages (from-to)346-353
Number of pages8
JournalMicrobes and Environments
Issue number3
Publication statusPublished - 2013


  • Cell sorter
  • Formate
  • Micro-colony
  • Nitrification
  • Uncultured bacteria

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics
  • Soil Science
  • Plant Science


Dive into the research topics of 'Isolation of Nitrospira belonging to sublineage II from a wastewater treatment plant'. Together they form a unique fingerprint.

Cite this