Isolation of replicational cue elements from a library of bent DNAs of Aspergillus oryzae

Takahiro Kusakabe; Yasushi Sugimoto; Yoshiko Hirota; Shigenobu Toné; Yutaka Kawaguchi; Katsumi Koga; Takashi Ohyama

doi:10.1023/A:1007076511814

Isolation of replicational cue elements from a library of bent DNAs of Aspergillus oryzae

Takahiro Kusakabe^*, Yasushi Sugimoto, Yoshiko Hirota, Shigenobu Toné, Yutaka Kawaguchi, Katsumi Koga, Takashi Ohyama

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

2 Citations (Scopus)

Abstract

Two fragments that could function as replicational cue elements were isolated from a genomic DNA digest of Aspergillus oryzae on the basis of abnormal behavior in polyacrylamide gel electrophoresis. The vector used in this study contained a scaffold-associated region of the Drosophila melanogaster ftz gene to provide nuclear retention. Neither fragment contained a yeast ARS consensus sequence or an eukaryotic topoisomerase II binding sequence. One of the fragments showed sequence homology with the mitochondrial replication origin of Candida utilis and a portion of mitochondrial DNA of Aspergillus nidulans. This plasmid carrying the cue fragment could also replicate in HeLa and NIH3T3 cells.

Original language	English
Pages (from-to)	13-19
Number of pages	7
Journal	Molecular Biology Reports
Volume	27
Issue number	1
DOIs	https://doi.org/10.1023/A:1007076511814
Publication status	Published - 2000
Externally published	Yes

Keywords

Autonomously replicating sequence
Fungal genome
Scaffold-associated region
Unusual DNA structure

ASJC Scopus subject areas

Molecular Biology
Genetics

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10.1023/A:1007076511814

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abstract = "Two fragments that could function as replicational cue elements were isolated from a genomic DNA digest of Aspergillus oryzae on the basis of abnormal behavior in polyacrylamide gel electrophoresis. The vector used in this study contained a scaffold-associated region of the Drosophila melanogaster ftz gene to provide nuclear retention. Neither fragment contained a yeast ARS consensus sequence or an eukaryotic topoisomerase II binding sequence. One of the fragments showed sequence homology with the mitochondrial replication origin of Candida utilis and a portion of mitochondrial DNA of Aspergillus nidulans. This plasmid carrying the cue fragment could also replicate in HeLa and NIH3T3 cells.",

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AU - Kusakabe, Takahiro

AU - Sugimoto, Yasushi

AU - Hirota, Yoshiko

AU - Toné, Shigenobu

AU - Kawaguchi, Yutaka

AU - Koga, Katsumi

AU - Ohyama, Takashi

PY - 2000

Y1 - 2000

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KW - Unusual DNA structure

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Isolation of replicational cue elements from a library of bent DNAs of Aspergillus oryzae

Abstract

Keywords

ASJC Scopus subject areas

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