TY - JOUR
T1 - Label-free detection of tumor markers using field effect transistor (FET)-based biosensors for lung cancer diagnosis
AU - Cheng, Shanshan
AU - Hideshima, Sho
AU - Kuroiwa, Shigeki
AU - Nakanishi, Takuya
AU - Osaka, Tetsuya
N1 - Funding Information:
This work was financially supported in part by COI STREAM (Center of Innovation Science and Technology based Radical Innovation and Entrepreneurship Program) from the Ministry of Education, Culture, Sports, Science and Technology (MEXT), Japan.
Publisher Copyright:
© 2015 Elsevier B.V. All rights reserved.
PY - 2015/6
Y1 - 2015/6
N2 - Detection of tumor markers is important for cancer diagnosis. Field effect transistor (FET) has been recognized as a powerful technique for label-free, sensitive, real-time, and multifunctional biosensing. Here, we developed FET biosensors that allow the label-free detection of cytokeratin fragment 21-1 (CYFRA 21-1) and neuron-specific enolase (NSE), useful tumor markers for lung cancer type differentiation. It was found that the FET biosensor was capable of quantitatively detecting these tumor markers in both phosphate-buffered saline and human serum. Additionally, we developed a multianalyte FET biosensor for the selective multiplexed detection of CYFRA 21-1 and NSE at the same time, by integrating two antibody types on the same chip, providing a step towards the realization of sensor arrays. The multianalyte FET biosensor, as described herein, will help for lung cancer differential diagnosis with advantages of simple and rapid detection procedures, low sample consumption, and low cost.
AB - Detection of tumor markers is important for cancer diagnosis. Field effect transistor (FET) has been recognized as a powerful technique for label-free, sensitive, real-time, and multifunctional biosensing. Here, we developed FET biosensors that allow the label-free detection of cytokeratin fragment 21-1 (CYFRA 21-1) and neuron-specific enolase (NSE), useful tumor markers for lung cancer type differentiation. It was found that the FET biosensor was capable of quantitatively detecting these tumor markers in both phosphate-buffered saline and human serum. Additionally, we developed a multianalyte FET biosensor for the selective multiplexed detection of CYFRA 21-1 and NSE at the same time, by integrating two antibody types on the same chip, providing a step towards the realization of sensor arrays. The multianalyte FET biosensor, as described herein, will help for lung cancer differential diagnosis with advantages of simple and rapid detection procedures, low sample consumption, and low cost.
KW - Field effect transistor
KW - Lung cancer differential diagnosis
KW - Multianalyte biosensor
KW - Tumor marker
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U2 - 10.1016/j.snb.2015.02.038
DO - 10.1016/j.snb.2015.02.038
M3 - Article
AN - SCOPUS:84923624026
SN - 0925-4005
VL - 212
SP - 329
EP - 334
JO - Sensors and Actuators, B: Chemical
JF - Sensors and Actuators, B: Chemical
ER -
