TY - JOUR
T1 - Lactobacillus equicursoris sp. nov., isolated from the faeces of a thoroughbred racehorse
AU - Morita, Hidetoshi
AU - Shimazu, Mitsuharu
AU - Shiono, Hiroshi
AU - Toh, Hidehiro
AU - Nakajima, Fumihiko
AU - Akita, Hiroaki
AU - Takagi, Misako
AU - Takami, Hideto
AU - Murakami, Masaru
AU - Masaoka, Toshio
AU - Tanabe, Soichi
AU - Hattori, Masahira
PY - 2010
Y1 - 2010
N2 - We previously isolated five strains of putative lactobacilli from the faeces of a thoroughbred horse (a 4-year-old male). Of the five strains, four were identified as members of existing Lactobacillus species; however, sequence analysis of the 16S rRNA gene revealed that the fifth isolate, DI70T, showed approximately 97% identity (1325/1366 bp) with the type strain of Lactobacillus delbrueckii. Therefore, we considered the possibility that DI70T represents a novel species of the genus Lactobacillus. Cells of strain DI70T were Gram-stain-positive, catalase-negative, non-spore forming, non-motile rods. In phylogenetic trees constructed on the basis of 16S rRNA gene sequences, strain DI70T formed a subcluster in the L. delbrueckii phylogenetic group and was closely related to L. delbrueckii, Lactobacillus crispatus and Lactobacillus jensenii. However, analysis of DNA-DNA relatedness showed that DI70T was genetically distinct from its phylogenetic relatives. The isolate also exhibited distinct biochemical and physiological characteristics when compared with its phylogenetic relatives. It required anaerobic conditions for growth on agar medium. The results indicate that isolate DI70T indeed represents a novel species of the genus Lactobacillus, for which we propose the name Lactobacillus equicursoris sp. nov. The type strain is DI70T (=JCM 14600T =DSM 19284T).
AB - We previously isolated five strains of putative lactobacilli from the faeces of a thoroughbred horse (a 4-year-old male). Of the five strains, four were identified as members of existing Lactobacillus species; however, sequence analysis of the 16S rRNA gene revealed that the fifth isolate, DI70T, showed approximately 97% identity (1325/1366 bp) with the type strain of Lactobacillus delbrueckii. Therefore, we considered the possibility that DI70T represents a novel species of the genus Lactobacillus. Cells of strain DI70T were Gram-stain-positive, catalase-negative, non-spore forming, non-motile rods. In phylogenetic trees constructed on the basis of 16S rRNA gene sequences, strain DI70T formed a subcluster in the L. delbrueckii phylogenetic group and was closely related to L. delbrueckii, Lactobacillus crispatus and Lactobacillus jensenii. However, analysis of DNA-DNA relatedness showed that DI70T was genetically distinct from its phylogenetic relatives. The isolate also exhibited distinct biochemical and physiological characteristics when compared with its phylogenetic relatives. It required anaerobic conditions for growth on agar medium. The results indicate that isolate DI70T indeed represents a novel species of the genus Lactobacillus, for which we propose the name Lactobacillus equicursoris sp. nov. The type strain is DI70T (=JCM 14600T =DSM 19284T).
UR - http://www.scopus.com/inward/record.url?scp=76849086045&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=76849086045&partnerID=8YFLogxK
U2 - 10.1099/ijs.0.009290-0
DO - 10.1099/ijs.0.009290-0
M3 - Article
C2 - 19648335
AN - SCOPUS:76849086045
SN - 1466-5026
VL - 60
SP - 109
EP - 112
JO - International Journal of Systematic and Evolutionary Microbiology
JF - International Journal of Systematic and Evolutionary Microbiology
IS - 1
ER -