Large scale microfluidic systems for sequential trapping, labeling and content extraction of single cells

Masao Noguchi; Takahiro Arakawa; Keiko Sumitomo; Yoshinori Yamaguchi; Shuichi Shoji

doi:10.1109/SENSOR.2009.5285403

Large scale microfluidic systems for sequential trapping, labeling and content extraction of single cells

Masao Noguchi^*, Takahiro Arakawa, Keiko Sumitomo, Yoshinori Yamaguchi, Shuichi Shoji

^*Corresponding author for this work

School of Fundamental Science and Engineering

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Abstract

Parallel and sequential manipulation of trapping, labeling and content extraction of single cell was realized using a 2-D simple flow channels. The proposed system consists of main channels, cell trapping pockets, drain channels and single cell content collection channels. We fabricated prototypes having 16 cell trapping pockets and 512 cell trapping pockets. Sequential all single cell handling for COS-1 cell was confirmed in 16 pockets type [1]. The sequential trapping and labeling were achieved in 512 pockets type. Capture rate for COS-1 cell was 83.2% and that for BAT cell was 70.3%. These devices are useful for high throughput single cell analysis.

Original language	English
Title of host publication	TRANSDUCERS 2009 - 15th International Conference on Solid-State Sensors, Actuators and Microsystems
Pages	628-631
Number of pages	4
DOIs	https://doi.org/10.1109/SENSOR.2009.5285403
Publication status	Published - 2009 Dec 11
Event	TRANSDUCERS 2009 - 15th International Conference on Solid-State Sensors, Actuators and Microsystems - Denver, CO, United States Duration: 2009 Jun 21 → 2009 Jun 25

Publication series

Name	TRANSDUCERS 2009 - 15th International Conference on Solid-State Sensors, Actuators and Microsystems

Conference

Conference	TRANSDUCERS 2009 - 15th International Conference on Solid-State Sensors, Actuators and Microsystems
Country/Territory	United States
City	Denver, CO
Period	09/6/21 → 09/6/25

Keywords

Single cell content extraction
Single cell labeling
Single cell manipulation
Single cell trapping

ASJC Scopus subject areas

Hardware and Architecture
Electrical and Electronic Engineering

Access to Document

10.1109/SENSOR.2009.5285403

Cite this

Noguchi, M., Arakawa, T., Sumitomo, K., Yamaguchi, Y., & Shoji, S. (2009). Large scale microfluidic systems for sequential trapping, labeling and content extraction of single cells. In TRANSDUCERS 2009 - 15th International Conference on Solid-State Sensors, Actuators and Microsystems (pp. 628-631). Article 5285403 (TRANSDUCERS 2009 - 15th International Conference on Solid-State Sensors, Actuators and Microsystems). https://doi.org/10.1109/SENSOR.2009.5285403

Large scale microfluidic systems for sequential trapping, labeling and content extraction of single cells. / Noguchi, Masao; Arakawa, Takahiro; Sumitomo, Keiko et al.
TRANSDUCERS 2009 - 15th International Conference on Solid-State Sensors, Actuators and Microsystems. 2009. p. 628-631 5285403 (TRANSDUCERS 2009 - 15th International Conference on Solid-State Sensors, Actuators and Microsystems).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Noguchi, M, Arakawa, T, Sumitomo, K, Yamaguchi, Y & Shoji, S 2009, Large scale microfluidic systems for sequential trapping, labeling and content extraction of single cells. in TRANSDUCERS 2009 - 15th International Conference on Solid-State Sensors, Actuators and Microsystems., 5285403, TRANSDUCERS 2009 - 15th International Conference on Solid-State Sensors, Actuators and Microsystems, pp. 628-631, TRANSDUCERS 2009 - 15th International Conference on Solid-State Sensors, Actuators and Microsystems, Denver, CO, United States, 09/6/21. https://doi.org/10.1109/SENSOR.2009.5285403

Noguchi M, Arakawa T, Sumitomo K, Yamaguchi Y, Shoji S. Large scale microfluidic systems for sequential trapping, labeling and content extraction of single cells. In TRANSDUCERS 2009 - 15th International Conference on Solid-State Sensors, Actuators and Microsystems. 2009. p. 628-631. 5285403. (TRANSDUCERS 2009 - 15th International Conference on Solid-State Sensors, Actuators and Microsystems). doi: 10.1109/SENSOR.2009.5285403

Noguchi, Masao ; Arakawa, Takahiro ; Sumitomo, Keiko et al. / Large scale microfluidic systems for sequential trapping, labeling and content extraction of single cells. TRANSDUCERS 2009 - 15th International Conference on Solid-State Sensors, Actuators and Microsystems. 2009. pp. 628-631 (TRANSDUCERS 2009 - 15th International Conference on Solid-State Sensors, Actuators and Microsystems).

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Large scale microfluidic systems for sequential trapping, labeling and content extraction of single cells

Abstract

Publication series

Conference

Keywords

ASJC Scopus subject areas

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