Ligand Exchange Reaction of Carbonylhemoglobin to Oxyhemoglobin in a Hemoglobin Liquid Membrane

Joo Eun Chung, Kenichi Hamada, Hiromi Sakai, Shinji Takeoka, Eishun Tsuchida*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

20 Citations (Scopus)


In order to change carbonylhemoglobin (HbCO) to oxyhemoglobin (Hb02), the photodissociation of HbCO and 02-binding to hemoglobin were studied using Hb liquid membrane system in O2 atmosphere. The reduction rate of HbCO increased with illuminance and saturated nearly at 2.0 • 105 lx. under a constant O2 pressure of 760 Torr. The reduction rate of HbCO was a 5-fold low when the partial O2 pressure was decreased from 760 to 0 Torr under the illuminance. In the static liquid membrane the exchanging rate of CO ligand in HbCO was decreased with the increment of the thickness of the liquid membrane, while the stirred liquid membrane showed a reverse result. The ligand exchange apparatus was made based on the obtained results, and the high flow rate of Hb solution enhanced the reduced amount of HbCO. Moreover, when the ligand exchange of Hb-vesicle dispersion was compared with an Hb solution, it proceeded more efficiently due to the encapsulation of concentrated Hb into a small compartment dispersed in a buffer solution.

Original languageEnglish
Pages (from-to)123-127
Number of pages5
Issue number2
Publication statusPublished - 1995

ASJC Scopus subject areas

  • Chemistry(all)
  • Chemical Engineering(all)


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