Lipase Production from Hydrocarbons by Trichosporon fermentans WU-C12 in the Presence of Surfactants

When Trichosporon fermentans WU-C12 was cultivated for 3-4 days in media containing 3% (v/v) kerosene and 0.8% (v/v) Tween 85, 3% (v/v) gas oil and 0.6% (v/v) Triton X-405, and 3% (v/v) liquid paraffin and 0.6% (v/v) Span 80, the extracellular lipase activities reached 58-62 U/ml, approximately 2-3 times as much as that without any addition of surfactant. The extracellular and total (intra-plus extracellular) lipase activities were affected by the combination of surfactants and petroleum products as the carbon sources.