Loading and unloading of molecular cargo by DNA-conjugated microtubule

Shu Taira; Yong Zhong Du; Yuich Hiratsuka; Taro Q.P. Uyeda; Noboru Yumoto; Masato Kodaka

doi:10.1002/bit.21618

Loading and unloading of molecular cargo by DNA-conjugated microtubule

Shu Taira, Yong Zhong Du, Yuich Hiratsuka, Taro Q.P. Uyeda, Noboru Yumoto, Masato Kodaka^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

33 Citations (Scopus)

Abstract

We developed a novel method to load and unload molecular cargos to and from microtubules (MTs) that move on kinesin-coated surfaces. Quantum dots (Qds) (molecular cargo) connected to 21-mer DNA can be selectively loaded on DNA-conjugated MTs through DNA hybridization. The average velocity of the Qd-loaded MTs (0.43 ± 0.06 μm s^-1 at 25°C) was comparable to that of control MTs. In addition, MTs conjugated with two types DNA sequences can achieve multiloading of Qds. To unload Qd molecular cargos from MTs, the DNA double helix connecting Qds to MTs were cleaved by an appropriate restriction enzyme. This biomolecular motors-based transport system should enable us to construct nanometer-scale devices such as nanobiosensor, nanofluidic system, or nanomachine.

Original language	English
Pages (from-to)	734-739
Number of pages	6
Journal	Biotechnology and bioengineering
Volume	99
Issue number	3
DOIs	https://doi.org/10.1002/bit.21618
Publication status	Published - 2008 Feb 15
Externally published	Yes

Keywords

DNA hybridization
Microtubule-kinesin pair
Nanobiomachine
Quantum dots
Restriction enzymes

ASJC Scopus subject areas

Biotechnology
Bioengineering
Applied Microbiology and Biotechnology

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10.1002/bit.21618

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title = "Loading and unloading of molecular cargo by DNA-conjugated microtubule",

abstract = "We developed a novel method to load and unload molecular cargos to and from microtubules (MTs) that move on kinesin-coated surfaces. Quantum dots (Qds) (molecular cargo) connected to 21-mer DNA can be selectively loaded on DNA-conjugated MTs through DNA hybridization. The average velocity of the Qd-loaded MTs (0.43 ± 0.06 μm s-1 at 25°C) was comparable to that of control MTs. In addition, MTs conjugated with two types DNA sequences can achieve multiloading of Qds. To unload Qd molecular cargos from MTs, the DNA double helix connecting Qds to MTs were cleaved by an appropriate restriction enzyme. This biomolecular motors-based transport system should enable us to construct nanometer-scale devices such as nanobiosensor, nanofluidic system, or nanomachine.",

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author = "Shu Taira and Du, {Yong Zhong} and Yuich Hiratsuka and Uyeda, {Taro Q.P.} and Noboru Yumoto and Masato Kodaka",

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T1 - Loading and unloading of molecular cargo by DNA-conjugated microtubule

AU - Taira, Shu

AU - Du, Yong Zhong

AU - Hiratsuka, Yuich

AU - Uyeda, Taro Q.P.

AU - Yumoto, Noboru

AU - Kodaka, Masato

PY - 2008/2/15

Y1 - 2008/2/15

N2 - We developed a novel method to load and unload molecular cargos to and from microtubules (MTs) that move on kinesin-coated surfaces. Quantum dots (Qds) (molecular cargo) connected to 21-mer DNA can be selectively loaded on DNA-conjugated MTs through DNA hybridization. The average velocity of the Qd-loaded MTs (0.43 ± 0.06 μm s-1 at 25°C) was comparable to that of control MTs. In addition, MTs conjugated with two types DNA sequences can achieve multiloading of Qds. To unload Qd molecular cargos from MTs, the DNA double helix connecting Qds to MTs were cleaved by an appropriate restriction enzyme. This biomolecular motors-based transport system should enable us to construct nanometer-scale devices such as nanobiosensor, nanofluidic system, or nanomachine.

AB - We developed a novel method to load and unload molecular cargos to and from microtubules (MTs) that move on kinesin-coated surfaces. Quantum dots (Qds) (molecular cargo) connected to 21-mer DNA can be selectively loaded on DNA-conjugated MTs through DNA hybridization. The average velocity of the Qd-loaded MTs (0.43 ± 0.06 μm s-1 at 25°C) was comparable to that of control MTs. In addition, MTs conjugated with two types DNA sequences can achieve multiloading of Qds. To unload Qd molecular cargos from MTs, the DNA double helix connecting Qds to MTs were cleaved by an appropriate restriction enzyme. This biomolecular motors-based transport system should enable us to construct nanometer-scale devices such as nanobiosensor, nanofluidic system, or nanomachine.

KW - DNA hybridization

KW - Microtubule-kinesin pair

KW - Nanobiomachine

KW - Quantum dots

KW - Restriction enzymes

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Loading and unloading of molecular cargo by DNA-conjugated microtubule

Abstract

Keywords

ASJC Scopus subject areas

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