Local spectrum measurement system for 5-aminolaevulinic acid induced protoporphyrin IX fluorescence

Takehiro Ando*, Masafumi Noguchi, Etsuko Kobayashi, Hongen Liao, Takashi Maruyama, Yoshihiro Muragaki, Hiroshi Iseki, Ichiro Sakuma

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingConference contribution

1 Citation (Scopus)

Abstract

We have developed a local spectrum measurement system for 5-Aminolaevulinic acid (5-ALA) induced Protoporphyrin IX (PpIX) fluorescence, which consists of a semiconductor LASER, a CCD spectrometer, a personal computer and a fluorescence measurement probe. Performance evaluation results showed that the probe has sufficient ability to measure PpIX fluorescence with a spatial resolution of about 0.8mm around the working-distance. We used this probe for measurement of resected brain tissues to evaluate discrimination ability of 5-ALA induced PpIX fluorescence. To compare spectrums with pathology, we used a tissue holder to freeze tissues keeping their shape. This procedure enables precise comparison each measured point with pathology. Thirteen samples of brain tissue were measured using the developed system, and the results showed that PpIX fluorescence could be detected in tumor lesion even less than 1mm in diameter. Although some samples shrank to about 80% of holder size, characterized shape enabled to compare spectrums with pathology.

Original languageEnglish
Title of host publicationWorld Congress on Medical Physics and Biomedical Engineering
Subtitle of host publicationDiagnostic Imaging
PublisherSpringer Verlag
Pages375-378
Number of pages4
Edition2
ISBN (Print)9783642038785
DOIs
Publication statusPublished - 2009
Externally publishedYes
EventWorld Congress on Medical Physics and Biomedical Engineering: Diagnostic Imaging - Munich, Germany
Duration: 2009 Sept 72009 Sept 12

Publication series

NameIFMBE Proceedings
Number2
Volume25
ISSN (Print)1680-0737

Conference

ConferenceWorld Congress on Medical Physics and Biomedical Engineering: Diagnostic Imaging
Country/TerritoryGermany
CityMunich
Period09/9/709/9/12

Keywords

  • Brain tumor
  • Fluorescence spectrum
  • Protoporphyrin IX

ASJC Scopus subject areas

  • Bioengineering
  • Biomedical Engineering

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