Localization of orexin-A-like immunoreactivity in prolactin cells in the bullfrog (Rana catesbeiana) pituitary

Toshiharu Yamamoto; Hirohumi Suzuki; Haruko Uemura; Kazutoshi Yamamoto; Sakaé Kikuyama

doi:10.1016/j.ygcen.2003.09.004

Localization of orexin-A-like immunoreactivity in prolactin cells in the bullfrog (Rana catesbeiana) pituitary

Toshiharu Yamamoto^*, Hirohumi Suzuki, Haruko Uemura, Kazutoshi Yamamoto, Sakaé Kikuyama

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

24 Citations (Scopus)

Abstract

Immunohistochemical techniques were employed to investigate the distribution of orexin-A-like immunoreactivity in the bullfrog (Rana catesbeiana) pituitary. Orexin-A-immunoreactive cells were scattered throughout the pars distalis. We found that these cells corresponded to the cells immunostained with antiserum against bullfrog prolactin (fPRL). Immunoelectron microscopic analysis indicated that an orexin-A-like substance coexisted with fPRL within secretory granules. Western blot analysis of bullfrog pituitary extract revealed that anti-human orexin-A antiserum labeled two separate bands which were not labeled with anti-fPRL antiserum. The present study has, for the first time, provided evidence of the intragranular colocalization of orexin-A-like and PRL immunoreactivities in the bullfrog pituitary.

Original language	English
Pages (from-to)	186-192
Number of pages	7
Journal	General and Comparative Endocrinology
Volume	135
Issue number	2
DOIs	https://doi.org/10.1016/j.ygcen.2003.09.004
Publication status	Published - 2004 Jan 15

Keywords

Colocalization
Frog
Immunocytochemistry
Orexin-A
Pituitary
Prolactin

ASJC Scopus subject areas

Endocrinology

Access to Document

10.1016/j.ygcen.2003.09.004

Cite this

@article{493f8f19870a423ea09ee6b008241f44,

title = "Localization of orexin-A-like immunoreactivity in prolactin cells in the bullfrog (Rana catesbeiana) pituitary",

abstract = "Immunohistochemical techniques were employed to investigate the distribution of orexin-A-like immunoreactivity in the bullfrog (Rana catesbeiana) pituitary. Orexin-A-immunoreactive cells were scattered throughout the pars distalis. We found that these cells corresponded to the cells immunostained with antiserum against bullfrog prolactin (fPRL). Immunoelectron microscopic analysis indicated that an orexin-A-like substance coexisted with fPRL within secretory granules. Western blot analysis of bullfrog pituitary extract revealed that anti-human orexin-A antiserum labeled two separate bands which were not labeled with anti-fPRL antiserum. The present study has, for the first time, provided evidence of the intragranular colocalization of orexin-A-like and PRL immunoreactivities in the bullfrog pituitary.",

keywords = "Colocalization, Frog, Immunocytochemistry, Orexin-A, Pituitary, Prolactin",

author = "Toshiharu Yamamoto and Hirohumi Suzuki and Haruko Uemura and Kazutoshi Yamamoto and Saka{\'e} Kikuyama",

year = "2004",

month = jan,

day = "15",

doi = "10.1016/j.ygcen.2003.09.004",

language = "English",

volume = "135",

pages = "186--192",

journal = "General and Comparative Endocrinology",

issn = "0016-6480",

publisher = "Academic Press Inc.",

number = "2",

}

TY - JOUR

T1 - Localization of orexin-A-like immunoreactivity in prolactin cells in the bullfrog (Rana catesbeiana) pituitary

AU - Yamamoto, Toshiharu

AU - Suzuki, Hirohumi

AU - Uemura, Haruko

AU - Yamamoto, Kazutoshi

AU - Kikuyama, Sakaé

PY - 2004/1/15

Y1 - 2004/1/15

N2 - Immunohistochemical techniques were employed to investigate the distribution of orexin-A-like immunoreactivity in the bullfrog (Rana catesbeiana) pituitary. Orexin-A-immunoreactive cells were scattered throughout the pars distalis. We found that these cells corresponded to the cells immunostained with antiserum against bullfrog prolactin (fPRL). Immunoelectron microscopic analysis indicated that an orexin-A-like substance coexisted with fPRL within secretory granules. Western blot analysis of bullfrog pituitary extract revealed that anti-human orexin-A antiserum labeled two separate bands which were not labeled with anti-fPRL antiserum. The present study has, for the first time, provided evidence of the intragranular colocalization of orexin-A-like and PRL immunoreactivities in the bullfrog pituitary.

AB - Immunohistochemical techniques were employed to investigate the distribution of orexin-A-like immunoreactivity in the bullfrog (Rana catesbeiana) pituitary. Orexin-A-immunoreactive cells were scattered throughout the pars distalis. We found that these cells corresponded to the cells immunostained with antiserum against bullfrog prolactin (fPRL). Immunoelectron microscopic analysis indicated that an orexin-A-like substance coexisted with fPRL within secretory granules. Western blot analysis of bullfrog pituitary extract revealed that anti-human orexin-A antiserum labeled two separate bands which were not labeled with anti-fPRL antiserum. The present study has, for the first time, provided evidence of the intragranular colocalization of orexin-A-like and PRL immunoreactivities in the bullfrog pituitary.

KW - Colocalization

KW - Frog

KW - Immunocytochemistry

KW - Orexin-A

KW - Pituitary

KW - Prolactin

UR - http://www.scopus.com/inward/record.url?scp=0346992140&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0346992140&partnerID=8YFLogxK

U2 - 10.1016/j.ygcen.2003.09.004

DO - 10.1016/j.ygcen.2003.09.004

M3 - Article

C2 - 14697304

AN - SCOPUS:0346992140

SN - 0016-6480

VL - 135

SP - 186

EP - 192

JO - General and Comparative Endocrinology

JF - General and Comparative Endocrinology

IS - 2

ER -

Localization of orexin-A-like immunoreactivity in prolactin cells in the bullfrog (Rana catesbeiana) pituitary

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this