TY - JOUR
T1 - Magnesium chloride and polyamine can differentiate mouse embryonic stem cells into trophectoderm or endoderm
AU - Tanase, Jun ichi
AU - Yokoo, Takehiro
AU - Matsumura, Yuuki
AU - Kinoshita, Makoto
AU - Kikuchi, Yo
AU - Suemori, Hirofumi
AU - Ohyama, Takashi
N1 - Publisher Copyright:
© 2016 Elsevier Inc.
PY - 2017/1/22
Y1 - 2017/1/22
N2 - Magnesium chloride and polyamines stabilize DNA and chromatin. Furthermore, they can induce nucleosome aggregation and chromatin condensation in vitro. To determine the effects of elevating the cation concentrations in the nucleus of a living cell, we microinjected various concentrations of mono-, di- and polyvalent cation solutions into the nuclei of mouse embryonic stem (ES) cells and traced their fates. Here, we show that an elevation of either MgCl2, spermidine or spermine concentration in the nucleus exerts a significant effect on mouse ES cells, and can differentiate a certain population of the cells into trophectoderm, a lineage that mouse ES cells do not normally generate, or endoderm. It is hypothesized that the cell differentiation was most probably caused by the condensation of chromatin including the Oct3/4 locus, which was induced by the elevated concentrations of these cations.
AB - Magnesium chloride and polyamines stabilize DNA and chromatin. Furthermore, they can induce nucleosome aggregation and chromatin condensation in vitro. To determine the effects of elevating the cation concentrations in the nucleus of a living cell, we microinjected various concentrations of mono-, di- and polyvalent cation solutions into the nuclei of mouse embryonic stem (ES) cells and traced their fates. Here, we show that an elevation of either MgCl2, spermidine or spermine concentration in the nucleus exerts a significant effect on mouse ES cells, and can differentiate a certain population of the cells into trophectoderm, a lineage that mouse ES cells do not normally generate, or endoderm. It is hypothesized that the cell differentiation was most probably caused by the condensation of chromatin including the Oct3/4 locus, which was induced by the elevated concentrations of these cations.
KW - Differentiation
KW - Magnesium ion
KW - Mouse ES cell
KW - Nuclear microinjection
KW - Polyamine
KW - Trophectoderm
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U2 - 10.1016/j.bbrc.2016.11.108
DO - 10.1016/j.bbrc.2016.11.108
M3 - Article
C2 - 27876565
AN - SCOPUS:85007179582
SN - 0006-291X
VL - 482
SP - 764
EP - 770
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 4
ER -