Abstract
The control of actin-myosin movement using a bacterial magnetic particle (BMP) which was synthesized intracellularly by magnetic bacteria was investigated. Myosin immobilized on the BMP (myosin-BMP conjugate) was prepared with heterobifunctional reagents, Sulfo-LC-SPDP (sulfosuccinimidyl 6-(3′-(2-pyridyldithio)-propionamido)hexanoate) and Sulfo-SMCC (sulfosuccinimidyl 4-(N-maleimidomethyl)cyclohexane-1-carboxylate). When a magnetic force was applied in the same direction as the myosin-BMP conjugate movement (negative magnetic force) at 22-23°C, the velocity increased from 2.0 μm s-1 to 7.5 μm s-1. When a magnetic force was applied in the opposite direction to the myosin-BMP conjugate movement (positive magnetic force), the velocity decreased from 10.7 μm s-1 to 0 μm s-1. These results indicate that the sliding force of the myosin-BMP conjugate caused by the actin-myosin interaction can be controlled using a neodymium-boron (Nd-B) magnet. It will be possible to develop a nano-sized actuator that can be controlled by the external magnetic force. This system could also be applicable to analysis of the dynamics of a single myosin molecule.
| Original language | English |
|---|---|
| Pages (from-to) | 121-124 |
| Number of pages | 4 |
| Journal | Materials Science and Engineering C |
| Volume | 5 |
| Issue number | 2 C |
| Publication status | Published - 1997 Dec |
| Externally published | Yes |
Keywords
- Actin cables
- Bacterial magnetic particle
- Chara corallina
- Myosin
ASJC Scopus subject areas
- Biomaterials