Measurement of Chemiluminescence from Neutrophils in a 96-Well Microplate using Lumi Box U-800 II

Takashi Kikuchi; Katsuhiko Suzuki; Tatsuya Abe; Hideki Satoh; Tetsu Endoh; Hiroko Hasegawa; Shigeyuki Nakaji; Kazuo Sugawara; Takashi Kumae

doi:10.1002/(sici)1099-1271(199705/06)12:3<149::aid-bio440>3.0.co;2-o

Measurement of Chemiluminescence from Neutrophils in a 96-Well Microplate using Lumi Box U-800 II

Takashi Kikuchi^*, Katsuhiko Suzuki, Tatsuya Abe, Hideki Satoh, Tetsu Endoh, Hiroko Hasegawa, Shigeyuki Nakaji, Kazuo Sugawara, Takashi Kumae

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

26 Citations (Scopus)

Abstract

We have developed a microplate photon counting system based on a cooled charge-coupled device (Lumi Box U-800 II) jointly with Maikurotekku Nition Company (Chiba, Japan). The system makes it possible to quantify chemiluminescence (CL) in a 96-well microplate automatically and simultaneously in a single experiment. We studied the measurement conditions for a luminol-dependent CL assay from neutrophils stimulated with opsonized zymosan (OZ) using this system. Parameters examined included the effect of OZ dose per well, mixing speed, mixing time and detection time on CL responses. The results indicated that this system allows the measurement of CL from phagocytes on a large number of samples using small amounts of sample and regents.

Original language	English
Pages (from-to)	149-153
Number of pages	5
Journal	Luminescence
Volume	12
Issue number	3
DOIs	https://doi.org/10.1002/(sici)1099-1271(199705/06)12:3<149::aid-bio440>3.0.co;2-o
Publication status	Published - 1997
Externally published	Yes

Keywords

CCD
Chemiluminescence
Luminol
Microplate
Neutrophils
Opsonized zymosan

ASJC Scopus subject areas

Biophysics
Chemistry (miscellaneous)

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10.1002/(sici)1099-1271(199705/06)12:3<149::aid-bio440>3.0.co;2-o

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title = "Measurement of Chemiluminescence from Neutrophils in a 96-Well Microplate using Lumi Box U-800 II",

abstract = "We have developed a microplate photon counting system based on a cooled charge-coupled device (Lumi Box U-800 II) jointly with Maikurotekku Nition Company (Chiba, Japan). The system makes it possible to quantify chemiluminescence (CL) in a 96-well microplate automatically and simultaneously in a single experiment. We studied the measurement conditions for a luminol-dependent CL assay from neutrophils stimulated with opsonized zymosan (OZ) using this system. Parameters examined included the effect of OZ dose per well, mixing speed, mixing time and detection time on CL responses. The results indicated that this system allows the measurement of CL from phagocytes on a large number of samples using small amounts of sample and regents.",

keywords = "CCD, Chemiluminescence, Luminol, Microplate, Neutrophils, Opsonized zymosan",

author = "Takashi Kikuchi and Katsuhiko Suzuki and Tatsuya Abe and Hideki Satoh and Tetsu Endoh and Hiroko Hasegawa and Shigeyuki Nakaji and Kazuo Sugawara and Takashi Kumae",

year = "1997",

doi = "10.1002/(sici)1099-1271(199705/06)12:3<149::aid-bio440>3.0.co;2-o",

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T1 - Measurement of Chemiluminescence from Neutrophils in a 96-Well Microplate using Lumi Box U-800 II

AU - Kikuchi, Takashi

AU - Suzuki, Katsuhiko

AU - Abe, Tatsuya

AU - Satoh, Hideki

AU - Endoh, Tetsu

AU - Hasegawa, Hiroko

AU - Nakaji, Shigeyuki

AU - Sugawara, Kazuo

AU - Kumae, Takashi

PY - 1997

Y1 - 1997

N2 - We have developed a microplate photon counting system based on a cooled charge-coupled device (Lumi Box U-800 II) jointly with Maikurotekku Nition Company (Chiba, Japan). The system makes it possible to quantify chemiluminescence (CL) in a 96-well microplate automatically and simultaneously in a single experiment. We studied the measurement conditions for a luminol-dependent CL assay from neutrophils stimulated with opsonized zymosan (OZ) using this system. Parameters examined included the effect of OZ dose per well, mixing speed, mixing time and detection time on CL responses. The results indicated that this system allows the measurement of CL from phagocytes on a large number of samples using small amounts of sample and regents.

AB - We have developed a microplate photon counting system based on a cooled charge-coupled device (Lumi Box U-800 II) jointly with Maikurotekku Nition Company (Chiba, Japan). The system makes it possible to quantify chemiluminescence (CL) in a 96-well microplate automatically and simultaneously in a single experiment. We studied the measurement conditions for a luminol-dependent CL assay from neutrophils stimulated with opsonized zymosan (OZ) using this system. Parameters examined included the effect of OZ dose per well, mixing speed, mixing time and detection time on CL responses. The results indicated that this system allows the measurement of CL from phagocytes on a large number of samples using small amounts of sample and regents.

KW - CCD

KW - Chemiluminescence

KW - Luminol

KW - Microplate

KW - Neutrophils

KW - Opsonized zymosan

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JO - Luminescence

JF - Luminescence

IS - 3

ER -

Measurement of Chemiluminescence from Neutrophils in a 96-Well Microplate using Lumi Box U-800 II

Abstract

Keywords

ASJC Scopus subject areas

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