Metabolic assay system for micropatterned contractile myotubes

K. Nagamine; Y. Ido; S. Sekine; T. Miyake; M. Kanzaki; M. Nisizawa

doi:10.1149/1.3484104

Metabolic assay system for micropatterned contractile myotubes

K. Nagamine^*, Y. Ido, S. Sekine, T. Miyake, M. Kanzaki, M. Nisizawa

^*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Abstract

Contractile C₂C₁₂ myotube line patterns embedded in a fibrin gel have been developed to afford a physiologically relevant and stable bioassay system. We found that the myotubes supported by an elastic fibrin gel maintained their line patterns and contractile activities for a longer period of time (one week) than myotubes adhered on a conventional culture dish. Now, we are investigating contraction-mediated translocation of the GLUT4 glucose transporter protein in myotubes using this system.

Original language	English
Title of host publication	Chemical Sensors 9 -and- MEMS/NEMS 9
Publisher	Electrochemical Society Inc.
Pages	35-39
Number of pages	5
Edition	8
ISBN (Electronic)	9781607681779
ISBN (Print)	9781566778275
DOIs	https://doi.org/10.1149/1.3484104
Publication status	Published - 2010
Externally published	Yes

Publication series

Name	ECS Transactions
Number	8
Volume	33
ISSN (Print)	1938-5862
ISSN (Electronic)	1938-6737

ASJC Scopus subject areas

Engineering(all)

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10.1149/1.3484104

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abstract = "Contractile C2C12 myotube line patterns embedded in a fibrin gel have been developed to afford a physiologically relevant and stable bioassay system. We found that the myotubes supported by an elastic fibrin gel maintained their line patterns and contractile activities for a longer period of time (one week) than myotubes adhered on a conventional culture dish. Now, we are investigating contraction-mediated translocation of the GLUT4 glucose transporter protein in myotubes using this system.",

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AU - Nagamine, K.

AU - Ido, Y.

AU - Sekine, S.

AU - Miyake, T.

AU - Kanzaki, M.

AU - Nisizawa, M.

PY - 2010

Y1 - 2010

N2 - Contractile C2C12 myotube line patterns embedded in a fibrin gel have been developed to afford a physiologically relevant and stable bioassay system. We found that the myotubes supported by an elastic fibrin gel maintained their line patterns and contractile activities for a longer period of time (one week) than myotubes adhered on a conventional culture dish. Now, we are investigating contraction-mediated translocation of the GLUT4 glucose transporter protein in myotubes using this system.

AB - Contractile C2C12 myotube line patterns embedded in a fibrin gel have been developed to afford a physiologically relevant and stable bioassay system. We found that the myotubes supported by an elastic fibrin gel maintained their line patterns and contractile activities for a longer period of time (one week) than myotubes adhered on a conventional culture dish. Now, we are investigating contraction-mediated translocation of the GLUT4 glucose transporter protein in myotubes using this system.

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BT - Chemical Sensors 9 -and- MEMS/NEMS 9

PB - Electrochemical Society Inc.

ER -

Metabolic assay system for micropatterned contractile myotubes

Abstract

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