Metabolites as clock hands: Estimation of internal body time using blood metabolomics

Hitoshi Iuchi, Rikuhiro G. Yamada, Hiroki R. Ueda

Research output: Chapter in Book/Report/Conference proceedingChapter


The circadian clock governs body time and regulates many physiological functions including sleep and wake cycles, body temperature, feeding, and hormone secretion. Alignment of drug dosing time to body time can maximize the pharmacological effect and minimize untoward effects. Therefore, a simple and robust method for estimating body time is important for drug efficacy or “chronotherapy”. We previously reported that a metabolite timetable could estimate body time with good accuracy. A metabolite timetable was constructed by profiling metabolites in human and mouse with capillary electrophoresis-mass spectrometry (CE-MS) and liquid chromatography–mass spectrometry (LC-MS). In this chapter, we describe practical methods to profile and identify oscillating metabolites.

Original languageEnglish
Title of host publicationNeuromethods
PublisherHumana Press Inc.
Number of pages10
Publication statusPublished - 2016
Externally publishedYes

Publication series

ISSN (Print)0893-2336
ISSN (Electronic)1940-6045


  • Capillary electrophoresis–mass spectrometry
  • Chronotherapy
  • Circadian
  • Liquid chromatography–mass spectrometry
  • Metabolomics

ASJC Scopus subject areas

  • Neuroscience(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Pharmacology, Toxicology and Pharmaceutics(all)
  • Psychiatry and Mental health


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