TY - JOUR
T1 - Microbial diversity of anammox bacteria enriched from different types of seed sludge in an anaerobic continuous-feeding cultivation reactor
AU - Date, Yasuhiro
AU - Isaka, Kazuichi
AU - Ikuta, Hajime
AU - Sumino, Tatsuo
AU - Kaneko, Naoya
AU - Yoshie, Sachiko
AU - Tsuneda, Satoshi
AU - Inamori, Yuhei
N1 - Funding Information:
This work was partly supported by Waseda University Grant for Special Research Projects (2005A-504).
Copyright:
Copyright 2009 Elsevier B.V., All rights reserved.
PY - 2009/3
Y1 - 2009/3
N2 - Enrichment of anammox bacteria from three types of seed sludge, sewage, digester, and nitrification sludges, was conducted using a nonwoven fabric carrier for immobilizing the anammox bacteria, and the microbial diversity of the enriched anammox culture was investigated. About four months later, simultaneous removals of ammonium and nitrite, and production of a small amount of nitrate, which is unique to the anammox reaction, were observed in all 3 sludge reactors. Results of 16S rRNA gene analysis indicated that anammox bacteria were cultivated and diversified in each sludge type. Moreover, the microbial diversity of anammox bacteria was higher in the enriched culture from sewage sludge compared to the other two types of seed sludge. Bacillus sp. coexisted in the anammox culture cultivated from sewage sludge. These results suggest that differences in the anammox community in the enriched culture were caused by differences in the type of seed sludge.
AB - Enrichment of anammox bacteria from three types of seed sludge, sewage, digester, and nitrification sludges, was conducted using a nonwoven fabric carrier for immobilizing the anammox bacteria, and the microbial diversity of the enriched anammox culture was investigated. About four months later, simultaneous removals of ammonium and nitrite, and production of a small amount of nitrate, which is unique to the anammox reaction, were observed in all 3 sludge reactors. Results of 16S rRNA gene analysis indicated that anammox bacteria were cultivated and diversified in each sludge type. Moreover, the microbial diversity of anammox bacteria was higher in the enriched culture from sewage sludge compared to the other two types of seed sludge. Bacillus sp. coexisted in the anammox culture cultivated from sewage sludge. These results suggest that differences in the anammox community in the enriched culture were caused by differences in the type of seed sludge.
KW - Anaerobic ammonium oxidation (anammox)
KW - Microbial diversity
KW - Nitrogen removal
KW - Nonwoven fabric carrier
KW - Phylogenetic analysis
KW - Reproducible enrichment
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U2 - 10.1016/j.jbiosc.2008.11.015
DO - 10.1016/j.jbiosc.2008.11.015
M3 - Article
C2 - 19269593
AN - SCOPUS:61349098950
SN - 1389-1723
VL - 107
SP - 281
EP - 286
JO - Journal of Bioscience and Bioengineering
JF - Journal of Bioscience and Bioengineering
IS - 3
ER -