Microbioassay of phenylalanine in blood sera with a lactate electrode

Isao Karube*, Tadashi Matsunaga, Nobuaki Teraoka, Shuichi Suzuki

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

38 Citations (Scopus)


Microbioassay of phenylalanine in sera is carried out by incubation with Leuconostoc mesenteroides (6 h) followed by use of a lactate electrode (immobilized lactate oxidase and an oxygen electrode) to measure the lactate produced. A linear relationship is obtained between the current decrease and the phenylalanine concentration between 0.75 and 6 × 10-7g ml-1 , with a standard deviation of 1.7 × 10-8 g ml-1 at the 3 × 10-7 g ml-1 level.

Original languageEnglish
Pages (from-to)271-276
Number of pages6
JournalAnalytica Chimica Acta
Issue number2
Publication statusPublished - 1980
Externally publishedYes

ASJC Scopus subject areas

  • Analytical Chemistry
  • Environmental Chemistry
  • Biochemistry
  • Spectroscopy


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