TY - GEN
T1 - Microchannel array for long term monitoring of multicellular filamentous cyanobacterium with controlled perfusion culture
AU - Kajiyama, Risa
AU - Ishihara, Junichi
AU - Kawai, Kentaro
AU - Iwasaki, Hideo
AU - Shoji, Shuichi
PY - 2011
Y1 - 2011
N2 - We designed and fabricated a microfluidic system, which has 128 microchannels for observation of muliticellular filamentous cyanobacteria, Anabaena sp. PCC 7120, under microscope for a long term with controlled perfusion culture. Using automated programming, we were able to monitor cell differentiation and growth of many filaments at the same time in this device. We are able to inject medium or reagent individually into each channel at any time during the experiment. Since more than 100 different filaments were cultured for 96 hours continuously, we succeeded in observing their morphogenesis clearly.
AB - We designed and fabricated a microfluidic system, which has 128 microchannels for observation of muliticellular filamentous cyanobacteria, Anabaena sp. PCC 7120, under microscope for a long term with controlled perfusion culture. Using automated programming, we were able to monitor cell differentiation and growth of many filaments at the same time in this device. We are able to inject medium or reagent individually into each channel at any time during the experiment. Since more than 100 different filaments were cultured for 96 hours continuously, we succeeded in observing their morphogenesis clearly.
KW - Cell differentiation
KW - Culture bacterium
KW - Cyanobacterium
KW - Microfluidic
UR - http://www.scopus.com/inward/record.url?scp=84883800566&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84883800566&partnerID=8YFLogxK
M3 - Conference contribution
AN - SCOPUS:84883800566
SN - 9781618395955
T3 - 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011
SP - 819
EP - 821
BT - 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011
T2 - 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011
Y2 - 2 October 2011 through 6 October 2011
ER -