Microfluidic electrochemiluminescence (ECL) integrated flow cell for portable fluorescence detection

Miho Tsuwaki; Jun Mizuno; Takashi Kasahara; Tomohiko Edura; Eri Kunisawa; Ryoichi Ishimatsu; Shigeyuki Matsunami; Toshihiko Imato; Chihaya Adachi; Shuichi Shoji

doi:10.1109/MEMSYS.2014.6765585

Microfluidic electrochemiluminescence (ECL) integrated flow cell for portable fluorescence detection

Miho Tsuwaki, Jun Mizuno, Takashi Kasahara, Tomohiko Edura, Eri Kunisawa, Ryoichi Ishimatsu, Shigeyuki Matsunami, Toshihiko Imato, Chihaya Adachi, Shuichi Shoji

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

6 Citations (Scopus)

Abstract

We propose a portable electrochemiluminescence (ECL)-induced fluorescence chip which consists of flow channels for fluorescence sample and multi-color emitting ECL excitation source. A prototype ECL-induced fluorescence chip was fabricated by conventional photolithography and bonding technique. Device performance was evaluated using ECL of rubrene as excitation source and resorufin as fluorescent dye. Fluorescence of 500 μM resorufin (600 nm) was successfully detected using 10 mM rubrene solution (560 nm) under the applied voltage of 4 V. The proposed principle is applicable for portable and on-demand multi fluorescence detection device using its freedom of choice for combination of the ECL light source.

Original language	English
Title of host publication	MEMS 2014 - 27th IEEE International Conference on Micro Electro Mechanical Systems
Publisher	Institute of Electrical and Electronics Engineers Inc.
Pages	108-111
Number of pages	4
ISBN (Print)	9781479935086
DOIs	https://doi.org/10.1109/MEMSYS.2014.6765585
Publication status	Published - 2014 Jan 1
Event	27th IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2014 - San Francisco, CA, United States Duration: 2014 Jan 26 → 2014 Jan 30

Publication series

Name	Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)
ISSN (Print)	1084-6999

Conference

Conference	27th IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2014
Country/Territory	United States
City	San Francisco, CA
Period	14/1/26 → 14/1/30

ASJC Scopus subject areas

Electronic, Optical and Magnetic Materials
Condensed Matter Physics
Mechanical Engineering
Electrical and Electronic Engineering

Access to Document

10.1109/MEMSYS.2014.6765585

Cite this

Tsuwaki, M., Mizuno, J., Kasahara, T., Edura, T., Kunisawa, E., Ishimatsu, R., Matsunami, S., Imato, T., Adachi, C., & Shoji, S. (2014). Microfluidic electrochemiluminescence (ECL) integrated flow cell for portable fluorescence detection. In MEMS 2014 - 27th IEEE International Conference on Micro Electro Mechanical Systems (pp. 108-111). [6765585] (Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)). Institute of Electrical and Electronics Engineers Inc.. https://doi.org/10.1109/MEMSYS.2014.6765585

Microfluidic electrochemiluminescence (ECL) integrated flow cell for portable fluorescence detection. / Tsuwaki, Miho; Mizuno, Jun; Kasahara, Takashi et al.
MEMS 2014 - 27th IEEE International Conference on Micro Electro Mechanical Systems. Institute of Electrical and Electronics Engineers Inc., 2014. p. 108-111 6765585 (Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Tsuwaki, M, Mizuno, J, Kasahara, T, Edura, T, Kunisawa, E, Ishimatsu, R, Matsunami, S, Imato, T, Adachi, C & Shoji, S 2014, Microfluidic electrochemiluminescence (ECL) integrated flow cell for portable fluorescence detection. in MEMS 2014 - 27th IEEE International Conference on Micro Electro Mechanical Systems., 6765585, Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS), Institute of Electrical and Electronics Engineers Inc., pp. 108-111, 27th IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2014, San Francisco, CA, United States, 14/1/26. https://doi.org/10.1109/MEMSYS.2014.6765585

Tsuwaki M, Mizuno J, Kasahara T, Edura T, Kunisawa E, Ishimatsu R et al. Microfluidic electrochemiluminescence (ECL) integrated flow cell for portable fluorescence detection. In MEMS 2014 - 27th IEEE International Conference on Micro Electro Mechanical Systems. Institute of Electrical and Electronics Engineers Inc. 2014. p. 108-111. 6765585. (Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)). doi: 10.1109/MEMSYS.2014.6765585

Tsuwaki, Miho ; Mizuno, Jun ; Kasahara, Takashi et al. / Microfluidic electrochemiluminescence (ECL) integrated flow cell for portable fluorescence detection. MEMS 2014 - 27th IEEE International Conference on Micro Electro Mechanical Systems. Institute of Electrical and Electronics Engineers Inc., 2014. pp. 108-111 (Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)).

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abstract = "We propose a portable electrochemiluminescence (ECL)-induced fluorescence chip which consists of flow channels for fluorescence sample and multi-color emitting ECL excitation source. A prototype ECL-induced fluorescence chip was fabricated by conventional photolithography and bonding technique. Device performance was evaluated using ECL of rubrene as excitation source and resorufin as fluorescent dye. Fluorescence of 500 μM resorufin (600 nm) was successfully detected using 10 mM rubrene solution (560 nm) under the applied voltage of 4 V. The proposed principle is applicable for portable and on-demand multi fluorescence detection device using its freedom of choice for combination of the ECL light source.",

author = "Miho Tsuwaki and Jun Mizuno and Takashi Kasahara and Tomohiko Edura and Eri Kunisawa and Ryoichi Ishimatsu and Shigeyuki Matsunami and Toshihiko Imato and Chihaya Adachi and Shuichi Shoji",

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AU - Tsuwaki, Miho

AU - Mizuno, Jun

AU - Kasahara, Takashi

AU - Edura, Tomohiko

AU - Kunisawa, Eri

AU - Ishimatsu, Ryoichi

AU - Matsunami, Shigeyuki

AU - Imato, Toshihiko

AU - Adachi, Chihaya

AU - Shoji, Shuichi

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N2 - We propose a portable electrochemiluminescence (ECL)-induced fluorescence chip which consists of flow channels for fluorescence sample and multi-color emitting ECL excitation source. A prototype ECL-induced fluorescence chip was fabricated by conventional photolithography and bonding technique. Device performance was evaluated using ECL of rubrene as excitation source and resorufin as fluorescent dye. Fluorescence of 500 μM resorufin (600 nm) was successfully detected using 10 mM rubrene solution (560 nm) under the applied voltage of 4 V. The proposed principle is applicable for portable and on-demand multi fluorescence detection device using its freedom of choice for combination of the ECL light source.

AB - We propose a portable electrochemiluminescence (ECL)-induced fluorescence chip which consists of flow channels for fluorescence sample and multi-color emitting ECL excitation source. A prototype ECL-induced fluorescence chip was fabricated by conventional photolithography and bonding technique. Device performance was evaluated using ECL of rubrene as excitation source and resorufin as fluorescent dye. Fluorescence of 500 μM resorufin (600 nm) was successfully detected using 10 mM rubrene solution (560 nm) under the applied voltage of 4 V. The proposed principle is applicable for portable and on-demand multi fluorescence detection device using its freedom of choice for combination of the ECL light source.

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Microfluidic electrochemiluminescence (ECL) integrated flow cell for portable fluorescence detection

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