Microtechnologies to fuel neurobiological research with nanometer precision

Cecilia A. Brunello; Ville Jokinen; Prasanna Sakha; Hideyuki Terazono; Fumimasa Nomura; Tomoyuki Kaneko; Sari E. Lauri; Sami Franssila; Claudio Rivera; Kenji Yasuda; Henri J. Huttunen

doi:10.1186/1477-3155-11-11

Microtechnologies to fuel neurobiological research with nanometer precision

Cecilia A. Brunello, Ville Jokinen, Prasanna Sakha, Hideyuki Terazono, Fumimasa Nomura, Tomoyuki Kaneko, Sari E. Lauri, Sami Franssila, Claudio Rivera, Kenji Yasuda, Henri J. Huttunen^*

^*Corresponding author for this work

Research output: Contribution to journal › Review article › peer-review

14 Citations (Scopus)

Abstract

The interface between engineering and molecular life sciences has been fertile ground for advancing our understanding of complex biological systems. Engineered microstructures offer a diverse toolbox for cellular and molecular biologists to direct the placement of cells and small organisms, and to recreate biological functions in vitro: cells can be positioned and connected in a designed fashion, and connectivity and community effects of cells studied. Because of the highly polar morphology and finely compartmentalized functions of neurons, microfabricated cell culture systems and related on-chip technologies have become an important enabling platform for studying development, function and degeneration of the nervous system at the molecular and cellular level. Here we review some of the compartmentalization techniques developed so far to highlight how high-precision control of neuronal connectivity allows new approaches for studying axonal and synaptic biology.

Original language	English
Article number	11
Journal	Journal of Nanobiotechnology
Volume	11
Issue number	1
DOIs	https://doi.org/10.1186/1477-3155-11-11
Publication status	Published - 2013 Apr 10
Externally published	Yes

Keywords

Axonal transport
Connectivity
Electrophysiology
Microfabrication
Microfluidics
Micropatterning
Neurobiology
Neurodegeneration
On-chip technology
Plasticity
Synaptogenesis

ASJC Scopus subject areas

Bioengineering
Medicine (miscellaneous)
Molecular Medicine
Biomedical Engineering
Applied Microbiology and Biotechnology
Pharmaceutical Science

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10.1186/1477-3155-11-11

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title = "Microtechnologies to fuel neurobiological research with nanometer precision",

abstract = "The interface between engineering and molecular life sciences has been fertile ground for advancing our understanding of complex biological systems. Engineered microstructures offer a diverse toolbox for cellular and molecular biologists to direct the placement of cells and small organisms, and to recreate biological functions in vitro: cells can be positioned and connected in a designed fashion, and connectivity and community effects of cells studied. Because of the highly polar morphology and finely compartmentalized functions of neurons, microfabricated cell culture systems and related on-chip technologies have become an important enabling platform for studying development, function and degeneration of the nervous system at the molecular and cellular level. Here we review some of the compartmentalization techniques developed so far to highlight how high-precision control of neuronal connectivity allows new approaches for studying axonal and synaptic biology.",

keywords = "Axonal transport, Connectivity, Electrophysiology, Microfabrication, Microfluidics, Micropatterning, Neurobiology, Neurodegeneration, On-chip technology, Plasticity, Synaptogenesis",

author = "Brunello, {Cecilia A.} and Ville Jokinen and Prasanna Sakha and Hideyuki Terazono and Fumimasa Nomura and Tomoyuki Kaneko and Lauri, {Sari E.} and Sami Franssila and Claudio Rivera and Kenji Yasuda and Huttunen, {Henri J.}",

note = "Funding Information: Funding from the Academy of Finland project #253223 and Strategic Japanese-Finnish Cooperative Program on “Biomaterials for medical application” by Japan Science and Technology Agency (JST) are acknowledged.",

year = "2013",

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doi = "10.1186/1477-3155-11-11",

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AU - Brunello, Cecilia A.

AU - Jokinen, Ville

AU - Sakha, Prasanna

AU - Terazono, Hideyuki

AU - Nomura, Fumimasa

AU - Kaneko, Tomoyuki

AU - Lauri, Sari E.

AU - Franssila, Sami

AU - Rivera, Claudio

AU - Yasuda, Kenji

AU - Huttunen, Henri J.

N1 - Funding Information: Funding from the Academy of Finland project #253223 and Strategic Japanese-Finnish Cooperative Program on “Biomaterials for medical application” by Japan Science and Technology Agency (JST) are acknowledged.

PY - 2013/4/10

Y1 - 2013/4/10

N2 - The interface between engineering and molecular life sciences has been fertile ground for advancing our understanding of complex biological systems. Engineered microstructures offer a diverse toolbox for cellular and molecular biologists to direct the placement of cells and small organisms, and to recreate biological functions in vitro: cells can be positioned and connected in a designed fashion, and connectivity and community effects of cells studied. Because of the highly polar morphology and finely compartmentalized functions of neurons, microfabricated cell culture systems and related on-chip technologies have become an important enabling platform for studying development, function and degeneration of the nervous system at the molecular and cellular level. Here we review some of the compartmentalization techniques developed so far to highlight how high-precision control of neuronal connectivity allows new approaches for studying axonal and synaptic biology.

AB - The interface between engineering and molecular life sciences has been fertile ground for advancing our understanding of complex biological systems. Engineered microstructures offer a diverse toolbox for cellular and molecular biologists to direct the placement of cells and small organisms, and to recreate biological functions in vitro: cells can be positioned and connected in a designed fashion, and connectivity and community effects of cells studied. Because of the highly polar morphology and finely compartmentalized functions of neurons, microfabricated cell culture systems and related on-chip technologies have become an important enabling platform for studying development, function and degeneration of the nervous system at the molecular and cellular level. Here we review some of the compartmentalization techniques developed so far to highlight how high-precision control of neuronal connectivity allows new approaches for studying axonal and synaptic biology.

KW - Axonal transport

KW - Connectivity

KW - Electrophysiology

KW - Microfabrication

KW - Microfluidics

KW - Micropatterning

KW - Neurobiology

KW - Neurodegeneration

KW - On-chip technology

KW - Plasticity

KW - Synaptogenesis

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DO - 10.1186/1477-3155-11-11

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Microtechnologies to fuel neurobiological research with nanometer precision

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Keywords

ASJC Scopus subject areas

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