TY - JOUR
T1 - Mitotic Exit Network controls the localization of Cdc14 to the Spindle Pole Body in Saccharomyces cerevisiae
AU - Yoshida, Satoshi
AU - Asakawa, Kazuhide
AU - Toh-e, Akio
N1 - Funding Information:
We thank Y. Kikuchi for a KRI1-myc strain, a mad2 strain, pTS plasmids, and comments on the manuscript. We also thank M. Shirayama and K. Nasmyth for cdc5 (msd2-1) and CDC14-myc strains and J.V. Kilmartin for a nud1-44 strain. We also thank A. Amon for communicating her results before publication. This work was supported by the grants for scientific research from the Ministry of Education, Culture, Sports, Science and Technology. S.Y. is the recipient of a Japan Society for the Promotion of Science (JSPS) Pre-doctoral Research Fellowship.
PY - 2002/6/4
Y1 - 2002/6/4
N2 - Budding yeast Cdc14 phosphatase plays essential roles in mitotic exit. Cdc14 is sequestered in the nucleolus by its inhibitor Net1/Cfi1 and is only released from the nucleolus during anaphase to inactivate mitotic CDK. It is believed that the mitotic exit network (MEN) is required for the release of Cdc14 from the nucleolus because liberation of Cdc14 by net1/cfi1 mutations bypasses the essential role of the MEN. But how the MEN residing at the spindle pole body (SPB) controls the association of Cdc14 with Net1/Cfi1 in the nucleolus is not yet understood [1, 2]. We found that Cdc14-5GFP was released from the nucleolus in the MEN mutants (tem1, cdc15, dbf2, and nud1), but not in the cdc5 cells during early anaphase. The Cdc14 liberation from the nucleolus was inhibited by the Mad2 checkpoint and by the Bub2 checkpoint in a different manner when microtubule organization was disrupted. We observed Cdc14-5GFP atthe SPB in addition to the nucleolus. The SPB localization of Cdc14 was significantly affected by the MEN mutations and the bub2 mutation. We conclude that Cdc14 is released from the nucleolus at the onset of anaphase in a CDC5-dependent manner and that MEN factors possibly regulate Cdc14 release from the SPB.
AB - Budding yeast Cdc14 phosphatase plays essential roles in mitotic exit. Cdc14 is sequestered in the nucleolus by its inhibitor Net1/Cfi1 and is only released from the nucleolus during anaphase to inactivate mitotic CDK. It is believed that the mitotic exit network (MEN) is required for the release of Cdc14 from the nucleolus because liberation of Cdc14 by net1/cfi1 mutations bypasses the essential role of the MEN. But how the MEN residing at the spindle pole body (SPB) controls the association of Cdc14 with Net1/Cfi1 in the nucleolus is not yet understood [1, 2]. We found that Cdc14-5GFP was released from the nucleolus in the MEN mutants (tem1, cdc15, dbf2, and nud1), but not in the cdc5 cells during early anaphase. The Cdc14 liberation from the nucleolus was inhibited by the Mad2 checkpoint and by the Bub2 checkpoint in a different manner when microtubule organization was disrupted. We observed Cdc14-5GFP atthe SPB in addition to the nucleolus. The SPB localization of Cdc14 was significantly affected by the MEN mutations and the bub2 mutation. We conclude that Cdc14 is released from the nucleolus at the onset of anaphase in a CDC5-dependent manner and that MEN factors possibly regulate Cdc14 release from the SPB.
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U2 - 10.1016/S0960-9822(02)00870-9
DO - 10.1016/S0960-9822(02)00870-9
M3 - Article
C2 - 12062061
AN - SCOPUS:0037018852
SN - 0960-9822
VL - 12
SP - 944
EP - 950
JO - Current Biology
JF - Current Biology
IS - 11
ER -