Modified ELISA for ultrasensitive diagnosis

Naoko Tsurusawa; Jyunhao Chang; Mayuri Namba; Daiki Makioka; Sou Yamura; Kanako Iha; Yuta Kyosei; Satoshi Watabe; Teruki Yoshimura; Etsuro Ito

doi:10.3390/jcm10215197

Modified ELISA for ultrasensitive diagnosis

Naoko Tsurusawa, Jyunhao Chang, Mayuri Namba, Daiki Makioka, Sou Yamura, Kanako Iha, Yuta Kyosei, Satoshi Watabe, Teruki Yoshimura, Etsuro Ito^*

^*Corresponding author for this work

School of Education

Research output: Contribution to journal › Review article › peer-review

23 Citations (Scopus)

Abstract

An enzyme-linked immunosorbent assay (ELISA) can be used for quantitative measurement of proteins, and improving the detection sensitivity to the ultrasensitive level would facilitate the diagnosis of various diseases. In the present review article, we first define the term ‘ultrasensitive’. We follow this with a survey and discussion of the current literature regarding modified ELISA methods with ultrasensitive detection and their application for diagnosis. Finally, we introduce our own newly devised system for ultrasensitive ELISA combined with thionicotinamide adenine dinucleotide cycling and its application for the diagnosis of infectious diseases and lifestylerelated diseases. The aim of the present article is to expand the application of ultrasensitive ELISAs in the medical and biological fields.

Original language	English
Article number	5197
Journal	Journal of Clinical Medicine
Volume	10
Issue number	21
DOIs	https://doi.org/10.3390/jcm10215197
Publication status	Published - 2021 Nov 1

Keywords

Diagnosis
Infectious disease
Lifestyle-related disease
Thio-NAD cycling
Ultrasensitive ELISA

ASJC Scopus subject areas

General Medicine

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.3390/jcm10215197

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title = "Modified ELISA for ultrasensitive diagnosis",

abstract = "An enzyme-linked immunosorbent assay (ELISA) can be used for quantitative measurement of proteins, and improving the detection sensitivity to the ultrasensitive level would facilitate the diagnosis of various diseases. In the present review article, we first define the term {\textquoteleft}ultrasensitive{\textquoteright}. We follow this with a survey and discussion of the current literature regarding modified ELISA methods with ultrasensitive detection and their application for diagnosis. Finally, we introduce our own newly devised system for ultrasensitive ELISA combined with thionicotinamide adenine dinucleotide cycling and its application for the diagnosis of infectious diseases and lifestylerelated diseases. The aim of the present article is to expand the application of ultrasensitive ELISAs in the medical and biological fields.",

keywords = "Diagnosis, Infectious disease, Lifestyle-related disease, Thio-NAD cycling, Ultrasensitive ELISA",

author = "Naoko Tsurusawa and Jyunhao Chang and Mayuri Namba and Daiki Makioka and Sou Yamura and Kanako Iha and Yuta Kyosei and Satoshi Watabe and Teruki Yoshimura and Etsuro Ito",

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month = nov,

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doi = "10.3390/jcm10215197",

language = "English",

volume = "10",

journal = "Journal of Clinical Medicine",

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T1 - Modified ELISA for ultrasensitive diagnosis

AU - Tsurusawa, Naoko

AU - Chang, Jyunhao

AU - Namba, Mayuri

AU - Makioka, Daiki

AU - Yamura, Sou

AU - Iha, Kanako

AU - Kyosei, Yuta

AU - Watabe, Satoshi

AU - Yoshimura, Teruki

AU - Ito, Etsuro

PY - 2021/11/1

Y1 - 2021/11/1

N2 - An enzyme-linked immunosorbent assay (ELISA) can be used for quantitative measurement of proteins, and improving the detection sensitivity to the ultrasensitive level would facilitate the diagnosis of various diseases. In the present review article, we first define the term ‘ultrasensitive’. We follow this with a survey and discussion of the current literature regarding modified ELISA methods with ultrasensitive detection and their application for diagnosis. Finally, we introduce our own newly devised system for ultrasensitive ELISA combined with thionicotinamide adenine dinucleotide cycling and its application for the diagnosis of infectious diseases and lifestylerelated diseases. The aim of the present article is to expand the application of ultrasensitive ELISAs in the medical and biological fields.

AB - An enzyme-linked immunosorbent assay (ELISA) can be used for quantitative measurement of proteins, and improving the detection sensitivity to the ultrasensitive level would facilitate the diagnosis of various diseases. In the present review article, we first define the term ‘ultrasensitive’. We follow this with a survey and discussion of the current literature regarding modified ELISA methods with ultrasensitive detection and their application for diagnosis. Finally, we introduce our own newly devised system for ultrasensitive ELISA combined with thionicotinamide adenine dinucleotide cycling and its application for the diagnosis of infectious diseases and lifestylerelated diseases. The aim of the present article is to expand the application of ultrasensitive ELISAs in the medical and biological fields.

KW - Diagnosis

KW - Infectious disease

KW - Lifestyle-related disease

KW - Thio-NAD cycling

KW - Ultrasensitive ELISA

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Modified ELISA for ultrasensitive diagnosis

Abstract

Keywords

ASJC Scopus subject areas

UN SDGs

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