Molecular cloning and chromosomal localization of the human thrombopoietin gene

Yoshiaki Sohma; Hiromichi Akahori; Naohiko Seki; Tada aki Hori; Kinya Ogami; Takashi Kato; Yoshihiro Shimada; Kazuo Kawamura; Hiroshi Miyazaki

doi:10.1016/0014-5793(94)01008-0

Molecular cloning and chromosomal localization of the human thrombopoietin gene

Yoshiaki Sohma, Hiromichi Akahori, Naohiko Seki, Tada aki Hori, Kinya Ogami, Takashi Kato, Yoshihiro Shimada, Kazuo Kawamura^*, Hiroshi Miyazaki

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

247 Citations (Scopus)

Abstract

The complete gene for human thrombopoietin (TPO) has been cloned by screening a human genomic library using human TPO cDNA as a probe. This gene is 6.2 kb in length and contains six exons and five introns. It is shown that the human genome contains a single copy of the human TPO gene according to Southern blotting analysis. The transcription initiation site was determined by S1 nuclease mapping. The human TPO gene expressed TPO activity when transfected into COS-1 cells. The human TPO gene has been mapped to chromosome 3q27 by in situ hybridization using a biotin-labeled probe.

Original language	English
Pages (from-to)	57-61
Number of pages	5
Journal	FEBS Letters
Volume	353
Issue number	1
DOIs	https://doi.org/10.1016/0014-5793(94)01008-0
Publication status	Published - 1994 Oct 10
Externally published	Yes

Keywords

Chromosome mapping
Genomic DNA
Thrombopoietin

ASJC Scopus subject areas

Biophysics
Structural Biology
Biochemistry
Molecular Biology
Genetics
Cell Biology

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10.1016/0014-5793(94)01008-0

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abstract = "The complete gene for human thrombopoietin (TPO) has been cloned by screening a human genomic library using human TPO cDNA as a probe. This gene is 6.2 kb in length and contains six exons and five introns. It is shown that the human genome contains a single copy of the human TPO gene according to Southern blotting analysis. The transcription initiation site was determined by S1 nuclease mapping. The human TPO gene expressed TPO activity when transfected into COS-1 cells. The human TPO gene has been mapped to chromosome 3q27 by in situ hybridization using a biotin-labeled probe.",

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AU - Sohma, Yoshiaki

AU - Akahori, Hiromichi

AU - Seki, Naohiko

AU - Hori, Tada aki

AU - Ogami, Kinya

AU - Kato, Takashi

AU - Shimada, Yoshihiro

AU - Kawamura, Kazuo

AU - Miyazaki, Hiroshi

PY - 1994/10/10

Y1 - 1994/10/10

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AB - The complete gene for human thrombopoietin (TPO) has been cloned by screening a human genomic library using human TPO cDNA as a probe. This gene is 6.2 kb in length and contains six exons and five introns. It is shown that the human genome contains a single copy of the human TPO gene according to Southern blotting analysis. The transcription initiation site was determined by S1 nuclease mapping. The human TPO gene expressed TPO activity when transfected into COS-1 cells. The human TPO gene has been mapped to chromosome 3q27 by in situ hybridization using a biotin-labeled probe.

KW - Chromosome mapping

KW - Genomic DNA

KW - Thrombopoietin

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Molecular cloning and chromosomal localization of the human thrombopoietin gene

Abstract

Keywords

ASJC Scopus subject areas

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